NK dendritic cells expanded in IL-15 exhibit antitumor responses in vivo Journal Article
| Authors: | Chaudhry, U. I.; Plitas, G.; Burt, B. M.; Kingham, T. P.; Raab, J. R.; DeMatteo, R. P. |
| Article Title: | NK dendritic cells expanded in IL-15 exhibit antitumor responses in vivo |
| Abstract: | NK dendritic cells (NKDC) are a novel subtype of DC with NK cell properties. IL-15 is a pleiotropic cytokine that plays an obligate role in the proliferation and survival of NK cells. We hypothesized that IL-15 is also essential for NKDC development. NKDC were nearly absent in IL-15-/- mice, but restored by administration of exogenous IL-15. Treatment of wild-type mice with IL-15 caused a 2- to 3-fold expansion of both NK cells and NKDC. After 7 days of culture with IL-15, sorted splenic NKDC expanded 10-fold while NK cells increased 5-fold. NKDC expanded in IL-15 retained their cytolytic capacity but lost the ability to stimulate naive T cells. Meanwhile, NKDC expanded in IL-15 produced 10 times more IFN-γ as fresh NKDC and conferred protection in a tumor prevention model. Thus, IL-15 is essential to the proliferation and survival of NKDC and IL-15 expanded NKDC possess antitumor properties. Copyright © 2007 by The American Association of Immunologists, Inc. |
| Keywords: | controlled study; nonhuman; neoplasms; t lymphocyte; animals; mice; mice, knockout; cell survival; cells, cultured; cell maturation; dendritic cell; animal experiment; animal model; in vivo study; cell differentiation; antineoplastic activity; cell line, tumor; dendritic cells; gamma interferon; natural killer cell; killer cells, natural; cytolysis; neoplasm transplantation; antigen-presenting cells; interleukin 15; apc protein; interleukin-15 |
| Journal Title: | Journal of Immunology |
| Volume: | 179 |
| Issue: | 7 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 2007-10-01 |
| Start Page: | 4654 |
| End Page: | 4660 |
| Language: | English |
| PUBMED: | 17878363 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 10" - "Export Date: 17 November 2011" - "CODEN: JOIMA" - "Source: Scopus" |
