| Abstract: |
We have isolated rare cells bearing the NK cell surface marker NK1.1, as well as the dendritic cell (DC) marker CD11c, from the spleen, liver, lymph nodes, and thymus of normal mice. These cells possess both NK cell and DC function because they can lyse tumor cells and subsequently present Ags to naive Ag-specific T cells. Interestingly, in response to IL-4 plus either IL-2 or CpG, NKDC produce more IFN-γ than do DC, or even NK cells. We determined that CpG, but not IL-2, induces NKDC to secrete IFN-γ via the antocrine effects of IL-12. In vivo, CpG dramatically increases the number of NKDC. Furthermore, NKDC induce greater Ag-specific T cell activation than do DC after adoptive transfer. Their unique ability to lyse tumor cells, present Ags, and secrete inflammatory cytokines suggests that NKDC may play a crucial role in linking innate and adaptive immunity. |
| Keywords: |
controlled study; nonhuman; lymph nodes; animal cell; mouse; animals; mice; mice, knockout; cell function; interleukin 2; spleen; dendritic cell; interleukin 4; mice, inbred balb c; mice, inbred c57bl; mice, transgenic; liver; antigen presentation; lymphocyte activation; dendritic cells; gamma interferon; thymus; thymus gland; cpg island; cpg islands; lymph node; natural killer cell; adoptive transfer; killer cells, natural; autocrine communication; cytotoxicity, immunologic; immunophenotyping; cytokine release; t lymphocyte activation; interleukin 12; tumor cell destruction; interleukin-12; glycoprotein p 15095; interferon type ii; interferon production
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