Spatially organized differentiation of mouse pluripotent stem cells on micropatterned surfaces Journal Article

  


Authors: Morgani, S. M.; Hadjantonakis, A. K.
Article Title: Spatially organized differentiation of mouse pluripotent stem cells on micropatterned surfaces
Abstract: Pluripotent stem cells (PSCs) are the in vitro counterpart of the pluripotent epiblast of the mammalian embryo with the capacity to generate all cell types of the adult organism. During development, the three definitive germ layers are specified and simultaneously spatially organized. In contrast, differentiating PSCs tend to generate cell fates in a spatially disorganized manner. This has limited the in vitro study of specific cell–cell interactions and patterning mechanisms that occur in vivo. Here we describe a protocol to differentiate mouse PSCs in a spatially organized manner on micropatterned surfaces. Micropatterned chips comprise many colonies of uniform size and geometry facilitating a robust quantitative analysis of patterned fate specification. Furthermore, multiple factors may be simultaneously manipulated with temporal accuracy to probe the dynamic interactions regulating these processes. The micropattern system is scalable, providing a valuable tool to generate material for large-scale analysis and biochemical experiments that require substantial amounts of starting material, difficult to obtain from early embryos. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
Keywords: nonhuman; animal cell; mouse; embryo; animal experiment; in vivo study; in vitro study; quantitative analysis; geometry; pluripotent stem cell; pluripotent stem cells; cell interaction; mesoderm; endoderm; differentiation; gastrulation; patterning; epiblast; ectoderm; primitive streak; escs; male; article; micropatterns; epilcs
Journal Title: Methods in Molecular Biology
Volume: 2214
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2021-01-01
Start Page: 41
End Page: 58
Language: English
DOI: 10.1007/978-1-0716-0958-3_4
PUBMED: 32944902
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091193094&doi=10.1007%2f978-1-0716-0958-3_4&partnerID=40&md5=97e6a1d90197b890e4af7225d3235641
Notes: Chapter 4 in "Epigenetic Reprogramming During Mouse Embryogenesis: Methods and Protocols" (ISBN: 978-1-0716-0957-6) -- Export Date: 1 October 2020 -- Source: Scopus
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MSK Authors
  1. Anna-Katerina Hadjantonakis
    192 Hadjantonakis
  2. Sophie Maria Christina Morgani
    10 Morgani
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