Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning Journal Article

   


Authors: Morgani, S. M.; Metzger, J. J.; Nichols, J.; Siggia, E. D.; Hadjantonakis, A. K.
Article Title: Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning
Abstract: During gastrulation epiblast cells exit pluripotency as they specify and spatially arrange the three germ layers of the embryo. Similarly, human pluripotent stem cells (PSCs) undergo spatially organized fate specification on micropatterned surfaces. Since in vivo validation is not possible for the human, we developed a mouse PSC micropattern system and, with direct comparisons to mouse embryos, reveal the robust specification of distinct regional identities. BMP, WNT, ACTIVIN and FGF directed mouse epiblast-like cells to undergo an epithelial-to-mesenchymal transition and radially pattern posterior mesoderm fates. Conversely, WNT, ACTIVIN and FGF patterned anterior identities, including definitive endoderm. By contrast, epiblast stem cells, a developmentally advanced state, only specified anterior identities, but without patterning. The mouse micropattern system offers a robust scalable method to generate regionalized cell types present in vivo, resolve how signals promote distinct identities and generate patterns, and compare mechanisms operating in vivo and in vitro and across species. © Morgani et al.
Keywords: controlled study; protein expression; nonhuman; animal cell; mouse; animal tissue; confocal microscopy; transforming growth factor beta; embryo; animal experiment; cell fate; cell differentiation; cell population; tissue section; immunocytochemistry; down regulation; upregulation; pluripotent stem cell; wnt protein; transcription factor sox2; fibroblast growth factor; transcription factor nanog; endoderm; gastrulation; intracellular signaling; ectoderm; epithelial mesenchymal transition; germ layer; cryosection; activin; female; article
Journal Title: eLife
Volume: 7
ISSN: 2050-084X
Publisher: eLife Sciences Publications Ltd.  
Date Published: 2018-02-07
Start Page: e32839
Language: English
DOI: 10.7554/eLife.32839
PROVIDER: scopus
PMCID: PMC5807051
PUBMED: 29412136
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043489779&doi=10.7554%2feLife.32839&partnerID=40&md5=eb567c1e26cd386ef8db6860912e4758
Notes: Article -- Export Date: 2 April 2018 -- Source: Scopus
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MSK Authors
  1. Anna-Katerina Hadjantonakis
    191 Hadjantonakis
  2. Sophie Maria Christina Morgani
    10 Morgani
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