Activation of the fructose 1,6-bisphosphatase gene by 1,25-dihydroxyvitamin D(3) during monocytic differentiation Journal Article
| Authors: | Solomon, D. H.; Raynal, M. C.; Tejwani, G. A.; Cayre, Y. E. |
| Article Title: | Activation of the fructose 1,6-bisphosphatase gene by 1,25-dihydroxyvitamin D(3) during monocytic differentiation |
| Abstract: | Cells from the human leukemia cell line HL-60 undergo terminal monocyte-like differentiation after exposure to either the active circulating form of vitamin D3, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], or phorbol 12-myristate 13-acetate. Little is known about the genes that regulate monocytic differentiation. Using clonal variant cells of HL-60 origin, we constructed a cDNA library enriched for genes that are induced by 1,25-(OH)2D3. We now report that in HL-60, the fructose 1,6-bisphosphatase (FBPase; D-fructose-1,6-bisphosphate 1-phosphohydrolase, EC 3.1.3.11) gene is activated during 1,25-(OH)2D3-induced monocytic differentiation. This gene encodes two closely related mRNAs; one, activated by 1,25-(OH)2D3 at an early stage of HL-60 differentiation, encodes a protein that has homology to mammalian FBPase, a key enzyme in gluconeogenesis, although it does not exhibit its classical enzymatic activity. A second mRNA is activated by 1,25-(OH)2D3 mainly in peripheral blood monocytes. This mRNA is present in kidney as a unique transcript and encodes a protein with FBPase activity. Our data also show that this FBPase-encoding mRNA can be activated during monocytic maturation since it was detected in human alveolar macrophages. |
| Keywords: | human cell; mammalia; cell differentiation; calcitriol; cell culture; monocyte; hormonal regulation; genetic regulation; cell strain hl 60; human; priority journal; fructose bisphosphatase |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 85 |
| Issue: | 18 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1988-09-01 |
| Start Page: | 6904 |
| End Page: | 6908 |
| Language: | English |
| DOI: | 10.1073/pnas.85.18.6904 |
| PUBMED: | 2842796 |
| PROVIDER: | scopus |
| PMCID: | PMC282087 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
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