Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells Journal Article

  


Authors: Bories, D.; Raynal, M. C.; Solomon, D. H.; Darzynkiewicz, Z.; Cayre, Y. E.
Article Title: Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells
Abstract: Cells from the human leukemia cell line HL-60 undergo terminal differentiation when exposed to inducing agents. Differentiation of these cells is always accompanied by withdrawal from the cell cycle. Here we describe the isolation of a cDNA encoding a novel serine protease that is present in HL-60 cells and is down-regulated during induced differentiation of these cells. We have named this protease myeloblastin. Down-regulation of myeloblastin mRNA occurs with both monocytic and granulocytic inducers. Myeloblastin mRNA is undetectable in fully differentiated HL-60 cells as well as in human peripheral blood monocytes. We found that regulation of myeloblastin mRNA in HL-60 cells is serum dependent. Inhibition of myeloblastin expression by an antisense oligodeoxynucleotide inhibits proliferation and induces differentiation of promyelocyte-like leukemia cells. © 1989.
Keywords: human cell; cell division; cell line; cell differentiation; transcription, genetic; tumor cells, cultured; leukemia, promyelocytic, acute; amino acid sequence; molecular sequence data; serine proteinase; serine endopeptidases; cell culture; rna, messenger; leukemia cell; dna, neoplasm; promyelocytic leukemia; base sequence; down regulation; myeloblastin; culture media; oligodeoxyribonucleotides; gene expression regulation, enzymologic; cell strain hl 60; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; genes, structural
Journal Title: Cell
Volume: 59
Issue: 6
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 1989-12-22
Start Page: 959
End Page: 968
Language: English
DOI: 10.1016/0092-8674(89)90752-6
PUBMED: 2598267
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024817730&doi=10.1016%2f0092-8674%2889%2990752-6&partnerID=40&md5=9dcbaedff53b5f8771cc2e63b50950d7
Notes: Article -- Export Date: 14 April 2020 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Zbigniew Darzynkiewicz
    61 Darzynkiewicz
Related MSK Work