1,25-dihydroxyvitamin D(3) transcriptionally regulates tumour necrosis factor mRNA during HL-60 cell differentiation Journal Article
| Authors: | Steffen, M.; Cayre, Y.; Manogue, K. R.; Moore, M. A. S. |
| Article Title: | 1,25-dihydroxyvitamin D(3) transcriptionally regulates tumour necrosis factor mRNA during HL-60 cell differentiation |
| Abstract: | We investigated TNF-α gene expression in HL-60 cells under the influence of 1,25(OH)2D3. Our results show that TNF-α gene expression is transcriptionally activated by 1,25(OH)2D3. Despite the increase of TNF-α mRNA, very little TNF protein is found in either supernatants or cell lysates of HL-60 cells cultured in the presence of the vitamin. Therefore 1,25(OH)2D3 is regulating the transcription of the TNF-α gene in HL-60 cells without affecting translation. Our data suggest that an additional signal is necessary under physiological conditions to control post-transcriptionally TNF-α protein synthesis and secretion. |
| Keywords: | human cell; gene expression; cell differentiation; transcription, genetic; calcitriol; tumor cells, cultured; gene expression regulation; tumor necrosis factor alpha; genetic engineering; cell culture; messenger rna; rna, messenger; tumor necrosis factor; cell strain hl 60; leukemia, myelocytic, acute; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Immunology |
| Volume: | 63 |
| Issue: | 1 |
| ISSN: | 0019-2805 |
| Publisher: | Wiley-Blackwell Publishing Ltd |
| Date Published: | 1988-01-01 |
| Start Page: | 43 |
| End Page: | 46 |
| Language: | English |
| PUBMED: | 3422222 |
| PROVIDER: | scopus |
| PMCID: | PMC1454706 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
