Synapse - Whole-exome sequencing analysis of the progression from non-low-grade ductal carcinoma in situ to invasive ductal carcinoma

Whole-exome sequencing analysis of the progression from non-low-grade ductal carcinoma in situ to invasive ductal carcinoma Journal Article

Authors:	Pareja, F.; Brown, D. N.; Lee, J. Y.; Da Cruz Paula, A.; Selenica, P.; Bi, R.; Geyer, F. C.; Gazzo, A.; da Silva, E. M.; Vahdatinia, M.; Stylianou, A. A.; Ferrando, L.; Wen, H. Y.; Hicks, J. B.; Weigelt, B.; Reis-Filho, J. S.
Article Title:	Whole-exome sequencing analysis of the progression from non-low-grade ductal carcinoma in situ to invasive ductal carcinoma
Abstract:	PURPOSE: Ductal carcinoma in situ (DCIS) is a nonobligate precursor of invasive breast cancer. Here, we sought to investigate the level of intralesion genetic heterogeneity in DCIS and the patterns of clonal architecture changes in the progression from DCIS to invasive disease. EXPERIMENTAL DESIGN: Synchronous DCIS (n = 27) and invasive ductal carcinomas of no special type (IDC-NSTs; n = 26) from 25 patients, and pure DCIS (n = 7) from 7 patients were microdissected separately and subjected to high-depth whole-exome (n = 56) or massively parallel sequencing targeting ≥410 key cancer-related genes (n = 4). Somatic genetic alterations, mutational signatures, clonal composition, and phylogenetic analyses were defined using validated computational methods. RESULTS: DCIS revealed genetic alterations similar to those of synchronously diagnosed IDC-NSTs and of non-related IDC-NSTs from The Cancer Genome Atlas (TCGA), whereas pure DCIS lacked PIK3CA mutations. Clonal decomposition and phylogenetic analyses based on somatic mutations and copy number alterations revealed that the mechanisms of progression of DCIS to invasive carcinoma are diverse, and that clonal selection might have constituted the mechanism of progression from DCIS to invasive disease in 28% (7/25) of patients. DCIS displaying a pattern of clonal selection in the progression to invasive cancer harbored higher levels of intralesion genetic heterogeneity than DCIS where no clonal selection was observed. CONCLUSIONS: Intralesion genetic heterogeneity is a common feature in DCIS synchronously diagnosed with IDC-NST. DCIS is a nonobligate precursor of IDC-NST, whose mechanisms of progression to invasive breast cancer are diverse and vary from case to case. ©2020 American Association for Cancer Research.
Journal Title:	Clinical Cancer Research
Volume:	26
Issue:	14
ISSN:	1078-0432
Publisher:	American Association for Cancer Research
Date Published:	2020-07-15
Start Page:	3682
End Page:	3693
Language:	English
DOI:	10.1158/1078-0432.Ccr-19-2563
PUBMED:	32220886
PROVIDER:	scopus
PMCID:	PMC7367727
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088276282&doi=10.1158%2f1078-0432.CCR-19-2563&partnerID=40&md5=33b73cdbc6070e49ecbb3841d1086b8a
Notes:	Article -- Export Date: 3 August 2020 -- Source: Scopus