Lobular carcinomas in situ display intralesion genetic heterogeneity and clonal evolution in the progression to invasive lobular carcinoma Journal Article


Authors: Lee, J. Y.; Schizas, M.; Geyer, F. C.; Selenica, P.; Piscuoglio, S.; Sakr, R. A.; Ng, C. K. Y.; Carniello, J. V. S.; Towers, R.; Giri, D. D.; de Andrade, V. P.; Papanastasiou, A. D.; Viale, A.; Harris, R. S.; Solit, D. B.; Weigelt, B.; Reis-Filho, J. S.; King, T. A.
Article Title: Lobular carcinomas in situ display intralesion genetic heterogeneity and clonal evolution in the progression to invasive lobular carcinoma
Abstract: Purpose: Lobular carcinoma in situ (LCIS) is a preinvasive mutations present in 81% of the lesions. Forty-two percent lesion of the breast. We sought to define its genomic landscape, (18/43) of LCIS were found to be clonally related to synchro-whether intralesion genetic heterogeneity is present in LCIS, nous DCIS and/or ILCs, with clonal evolutionary patterns and the clonal relatedness between LCIS and invasive breast indicative of clonal selection and/or parallel/branched pro-cancers. gression. Intralesion genetic heterogeneity was higher among Experimental Design: We reanalyzed whole-exome LCIS clonally related to DCIS/ILC than in those nonclonally sequencing (WES) data and performed a targeted amplicon related to DCIS/ILC. A shift from aging to APOBEC-related sequencing validation of mutations identified in 43 LCIS and mutational processes was observed in the progression from 27 synchronous more clinically advanced lesions from 24 LCIS to DCIS and/or ILC in a subset of cases. patients [9 ductal carcinomas in situ (DCIS), 13 invasive Conclusions: Our findings support the contention that lobular carcinomas (ILC), and 5 invasive ductal carcinomas LCIS has a repertoire of somatic genetic alterations similar to (IDC)]. Somatic genetic alterations, mutational signatures, that of ILCs, and likely constitutes a nonobligate precursor of clonal composition, and phylogenetic trees were defined using breast cancer. Intralesion genetic heterogeneity is observed in validated computational methods. LCIS and should be considered in studies aiming to develop Results: WES of 43 LCIS lesions revealed a genomic profile biomarkers of progression from LCIS to more advanced similar to that previously reported for ILCs, with CDH1 lesions. © 2018 American Association for Cancer Research.
Journal Title: Clinical Cancer Research
Volume: 25
Issue: 2
ISSN: 1078-0432
Publisher: American Association for Cancer Research  
Date Published: 2019-01-15
Start Page: 674
End Page: 686
Language: English
DOI: 10.1158/1078-0432.Ccr-18-1103
PUBMED: 30185420
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 1 February 2019 -- Source: Scopus
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MSK Authors
  1. David Solit
    421 Solit
  2. Dilip D Giri
    122 Giri
  3. Tari King
    163 King
  4. Rita Sakr
    59 Sakr
  5. Agnes Viale
    203 Viale
  6. Victor Piana De Andrade
    25 Andrade
  7. Russell Towers
    12 Towers
  8. Britta Weigelt
    258 Weigelt
  9. Jorge Sergio Reis
    283 Reis
  10. Kiu Yan Charlotte Ng
    152 Ng
  11. Michail Schizas
    15 Schizas
  12. Ju Youn Lee
    5 Lee