A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing Journal Article
| Authors: | Alexeeva, V.; Aydin, I. T.; Schaniel, C.; Stranahan, A. W.; D'Souza, S. L.; Bieker, J. J. |
| Article Title: | A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing |
| Abstract: | Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and ß-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and ß-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin. © 2020 The Author(s) |
| Keywords: | controlled study; unclassified drug; human cell; stop codon; polymerase chain reaction; gene expression; beta iii tubulin; beta tubulin; nuclear protein; green fluorescent protein; cell differentiation; hemoglobin beta chain; telomerase reverse transcriptase; real time polymerase chain reaction; hematopoietic stem cell; cell marker; karyotyping; mesoderm; octamer transcription factor 4; transcription factor sox2; genomic dna; transcription factor nanog; endoderm; alpha fetoprotein; smooth muscle actin; southern blotting; ectoderm; short tandem repeat; chromosome 20; transcription activator like effector nuclease; chromosome duplication; embryoid body; human; male; priority journal; article; gene editing; tuj1 protein; stem cell line; human embryonic stem cell; embryonal cell line; op9 cell line; wae001 a 2 cell line |
| Journal Title: | Stem Cell Research |
| Volume: | 45 |
| ISSN: | 1873-5061 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2020-05-01 |
| Start Page: | 101837 |
| Language: | English |
| DOI: | 10.1016/j.scr.2020.101837 |
| PUBMED: | 32413789 |
| PROVIDER: | scopus |
| PMCID: | PMC7297435 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2020 -- Source: Scopus |
