Forced expression of HoxB4 enhances hematopoietic differentiation by human embryonic stem cells Journal Article
| Authors: | Lee, G. S.; Kim, B. S.; Sheih, J. H.; Moore, M. A. S. |
| Article Title: | Forced expression of HoxB4 enhances hematopoietic differentiation by human embryonic stem cells |
| Abstract: | HoxB4 has been shown to enhance hematopoietic engraftment by hematopoietic stem cells (HSC) from differentiating mouse embryonic stem cell (mESC) cultures. Here we examined the effect of ectopic expression of HoxB4 in differentiated human embryonic stem cells (hESCs). Stable HoxB4-expressing hESCs were established by lentiviral transduction, and the forced expression of HoxB4 did not affect stem cell features. HoxB4-expressing hESC-derived CD34+ cells generated higher numbers of erythroid and blast-like colonies than controls. The number of CD34+ cells increased but CD45+ and KDR+ cell numbers were not significantly affected. When the hESC derived CD34+ cells were transplanted into NOD/ SCIDβ2m-/- mice, the ectopic expression of HoxB4 did not alter their repopulating capacity. Our findings show that overexpression of HoxB4 in differentiating hESCs increases hematopoietic colony formation and hematopoietic cell formation in vitro, but does not affect in vivo repopulation in adult mice hosts. © KSMCB 2008. |
| Keywords: | controlled study; protein expression; unclassified drug; human cell; nonhuman; protein function; animal cell; mouse; animals; mice; mus; cd34 antigen; gene overexpression; gene expression; embryonic stem cell; cell line; hematopoietic stem cell transplantation; cell differentiation; homeodomain proteins; in vitro study; mice, scid; cell population; vasculotropin receptor 2; viral gene delivery system; genetic transduction; transcription factors; transduction, genetic; lentivirus; lentivirus vector; hematopoietic cell; embryonic stem cells; cell count; hematopoiesis; homeodomain protein; antigens, cd34; scid mouse; cd45 antigen; colony forming cell; human embryonic stem cells; hematopoietic; hoxb4; protein hoxb4 |
| Journal Title: | Molecules and Cells |
| Volume: | 25 |
| Issue: | 4 |
| ISSN: | 1016-8478 |
| Publisher: | Ksmcb 2008 |
| Date Published: | 2008-06-30 |
| Start Page: | 487 |
| End Page: | 493 |
| Language: | English |
| PUBMED: | 18511880 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 6" - "Export Date: 17 November 2011" - "CODEN: MOCEE" - "Source: Scopus" |
