Hematopoietic cells Journal Article


Authors: Moore, M. A. S.; Shieh, J.H.; Lee, G.
Article Title: Hematopoietic cells
Abstract: Murine embryonic stem cells (mESC) readily form embryoid bodies (EBs) that exhibit hematopoietic differentiation. Methods based on EB formation or ESC coculture with murine bone marrow stromal cell lines have revealed pathways of both primitive and definitive hematopoietic differentiation progressing from primitive mesoderm via hemangioblasts to endothelium and hematopoietic stem and progenitor cells. The addition of specific hematopoietic growth factors and morphogens to these cultures enhances the generation of neutrophils, macrophages, megakaryocyte/platelets, and hemoglobinized mature red cells. In addition, selective culture systems have been developed to support differentiation into mature T lymphocytes, natural killer cells, B cells, and dendritic cells. In most cases, culture systems have been developed that support equivalent differentiation of various human ESC (hESC). The major obstacle to translation of ESC hematopoietic cultures to clinical relevance has been the general inability to produce hematopoietic stem cells (HSC) that can engraft adult, irradiated recipients. In this context, the pattern of ES hematopoietic development mirrors the yolk sac phase of hematopoiesis that precedes the appearance of engraftable HSC in the aorta-gonad-mesonephros region. Genetic manipulation of mESC hematopoietic progeny by upregulation of HOXB4 or STAT5 has led to greatly enhanced long- or short-term multilineage hematopoietic engraftment, suggesting that genetic or epigenetic manipulation of these pathways may lead to functional HSC generation from hESC.
Keywords: dendritic cells; es cells; expression; embryonic stem-cells; in-vitro differentiation; directed differentiation; bone-marrow; yolk-sac; ectopic hoxb4; marrow stromal cells; definitive hematopoiesis
Journal Title: Methods in Enzymology
Volume: 418
ISSN: 0076-6879
Publisher: Academic Press  
Publication Place: San Diego
Date Published: 2006-01-01
Start Page: 208
End Page: 242
Language: English
ACCESSION: WOS:000242859800013
PROVIDER: wos
PUBMED: 17141038
DOI: 10.1016/s0076-6879(06)18013-1
Notes: Chapter 13 in "Embryonic Stem Cells" (ISBN: 978-0-12-373648-2) - Review; Book Chapter - 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA - Source: Wos
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  1. Jae-Hung Shieh
    76 Shieh
  2. Gabsang Lee
    21 Lee
  3. Malcolm A S Moore
    549 Moore