Derivation of engraftable skeletal myoblasts from human embryonic stem cells Journal Article
| Authors: | Barberi, T.; Bradbury, M.; Dincer, Z.; Panagiotakos, G.; Socci, N. D.; Studer, L. |
| Article Title: | Derivation of engraftable skeletal myoblasts from human embryonic stem cells |
| Abstract: | Human embryonic stem cells (hESCs) are a promising source for cell therapy in degenerative diseases. A key step in establishing the medical potential of hESCs is the development of techniques for the conversion of hESCs into tissue-restricted precursors suitable for transplantation. We recently described the derivation of multipotent mesenchymal precursors from hESCs. Nevertheless, our previous study was limited by the requirement for mouse feeders and the lack of in vivo data. Here we report a stroma-free induction system for deriving mesenchymal precursors. Selective culture conditions and fluorescence-activated cell sorting (FACS)-mediated purification yielded multipotent mesenchymal precursors and skeletal myoblasts. Skeletal muscle cells undergo in vitro maturation resulting in myotube formation and spontaneous twitching. We found that hESC-derived skeletal myoblasts were viable after transplantation into the tibialis anterior muscle of SCID/Beige mice, as assessed by bioluminescence imaging. Lack of teratoma formation and evidence of long-term myoblast engraftment suggests considerable potential for future therapeutic applications. © 2007 Nature Publishing Group. |
| Keywords: | controlled study; nonhuman; animal cell; mouse; animals; mice; animal tissue; cell viability; mus; cell maturation; embryonic stem cell; animal experiment; stem cell transplantation; in vitro study; cell culture techniques; mesenchymal stem cell; embryonic stem cells; teratoma; stroma cell; stromal cells; multipotent stem cell; mesoderm; cell separation; bioluminescence; myoblast; myotube; myoblasts; neurodegenerative diseases; purification; fluorescence activated cell sorter; tibialis anterior muscle |
| Journal Title: | Nature Medicine |
| Volume: | 13 |
| Issue: | 5 |
| ISSN: | 1078-8956 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2007-05-01 |
| Start Page: | 642 |
| End Page: | 648 |
| Language: | English |
| DOI: | 10.1038/nm1533 |
| PUBMED: | 17417652 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 63" - "Export Date: 17 November 2011" - "CODEN: NAMEF" - "Source: Scopus" |
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