Growth hormone-induced alteration of morphology and tubulin expression in 3T3 preadipose cells Journal Article
| Authors: | Guller, S.; Corin, R. E.; Wu, K. Y.; Sonenberg, M. |
| Article Title: | Growth hormone-induced alteration of morphology and tubulin expression in 3T3 preadipose cells |
| Abstract: | Effects of growth hormone on morphology and cytoskeletal protein expression were examined in 3T3-F442A preadipocytes in serum-free medium. Between 2 and 5 days of culture 2 nM methionyl human growth hormone converted 3T3-F442A cells from a flat fibroblastic morphology to a rounded form with numerous membrane convolutions. Growth hormone treated cultures manifested a 30-40% reduction in cell volume. Growth hormone induced changes in morphology and volume preceded and were independent of lipogenesis. In cells treated with growth hormone, expression of α and β-tubulin as determined by Western blotting was found to increase approximately 50% within 72 h as compared to untreated cells. After 7 days, tubulin levels in growth hormone treated cells were approximately 40% of control levels. This indicated that morphological changes and alteration of tubulin expression were signatures of growth hormone action on 3T3-F442A cells. © 1989. |
| Keywords: | nonhuman; animal cell; mouse; cells, cultured; cell structure; gene expression; growth hormone; gene expression regulation; blotting, western; cell culture; fibroblasts; tubulin; cell strain 3t3; adipose tissue; cytoskeleton protein; cytoskeletal proteins; microscopy, electron, scanning; cell volume; proadipocyte; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 163 |
| Issue: | 2 |
| ISSN: | 0006-291X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1989-09-15 |
| Start Page: | 895 |
| End Page: | 901 |
| Language: | English |
| DOI: | 10.1016/0006-291x(89)92306-1 |
| PUBMED: | 2783130 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
