Effect of human growth hormone and insulin on [(3)H]thymidine incorporation, cell cycle progression, and cyclin D expression in 3T3-F442A preadipose cells Journal Article
| Authors: | Tang, B.; Jeoung, D. I.; Sonenberg, M. |
| Article Title: | Effect of human growth hormone and insulin on [(3)H]thymidine incorporation, cell cycle progression, and cyclin D expression in 3T3-F442A preadipose cells |
| Abstract: | Cellular growth- and cyclin D expression-regulating activities of GH and insulin were investigated in 3T3-F442A preadipose cells under serum-free culture conditions. The present report provides evidence that the proliferative potential of 3T3-F442A cells is reduced by GH in a time- and concentration-dependent manner based on [3H]thymidine incorporation assay and cell cycle analysis. In contrast, treatment of 3T3-F442A cells with insulin resulted in cellular proliferation. The insulin-induced proliferation of 3T3-F442A cells was diminished in the presence of GH. In an effort to define biochemical events relevant to the regulatory activities of GH and insulin on the proliferation of 3T3-F442A cells, the effects of these peptides on the expression of cyclin D were studied using Western blotting. Treatment of 3T3-F442A cells with insulin led to an increase in cyclin D expression relative to that in untreated cells. The insulin-elicited expression of cyclin D was time and dose dependent. In addition, the ability of insulin to induce cyclin D expression was reduced by GH. Our experimental results indicate that proliferation of 3T3-F442A cells was regulated by GH and insulin. The regulatory effects of GH and insulin are mediated at least in part by the alternating expression of cyclin D protein. © 1995 by The Endocrine Society. |
| Keywords: | unclassified drug; dose response; nonhuman; dna synthesis; cell proliferation; animal cell; mouse; animal; mice; cell cycle; gene expression; cell growth; growth hormone; blotting, western; dna; oncogene proteins; human growth hormone; insulin; cycline; cyclin d1; cyclins; s phase; 3t3 cells; cyclin d; proadipocyte; human; priority journal; article; support, u.s. gov't, p.h.s.; bovine insulin |
| Journal Title: | Endocrinology |
| Volume: | 136 |
| Issue: | 7 |
| ISSN: | 0013-7227 |
| Publisher: | Endocrine Society |
| Date Published: | 1995-07-01 |
| Start Page: | 3062 |
| End Page: | 3069 |
| Language: | English |
| DOI: | 10.1210/endo.136.7.7789333 |
| PUBMED: | 7789333 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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