Molecular mapping of a new public HLA class I epitope shared by all HLA-B and HLA-C antigens and defined by a monoclonal antibody Journal Article
| Authors: | Trapani, J. A.; Mizuno, S.; Kang, S. H.; Yang, S. Y.; Dupont, B. |
| Article Title: | Molecular mapping of a new public HLA class I epitope shared by all HLA-B and HLA-C antigens and defined by a monoclonal antibody |
| Abstract: | It has previously been shown that a mouse monoclonal antibody, designated 4E, reacts with an epitope common to all HLA-B and -C antigens and those of the HLA-Aw19 cross-reactive group, namely, HLA-A29,-A30, -A31, -A32, -Aw33, and -Aw74. In order to pinpoint the amino acid residues which comprise the public specificity recognized by 4E, an HLA-A29 cDNA clone was isolated and its predicted amino acid sequence compared with those of other cloned HLA class I genes. The isolated HLA-A29 cDNA corresponded to the rarer of the two A29 variant alleles, A29.1. Two amino acid residues of HLA-A29.1, gln-144 and arg-151, were found in all 24 HLA-B and HLA-C alleles examined but were present in only one of 15 HLA-A alleles for which sequence data are available. Importantly, this exceptional allele was HLA-A32, another member of the HLA-Aw19 cross-reactive group. Gln-144 and arg-151 should be capable of jointly contributing to the binding site for 4E, as they are situated in successive alpha-helical subregions and are predicted to be juxtaposed in the three-dimensional HLA molecule. Four other residues in the first or second external domains of HLA-A29.1 (thr-9, leu-62, gln-63, and his-102) were unique among the HLA-A alleles, but none of these was found in corresponding positions of HLA-B or -C alleles and thus failed to correlate with presence or absence of the 4E determinant. These observations are consistent with the notion that gln-144 and arg-151 define a determinant common to HLA-B, HLA-C, and the HLA-Awl9 cross-reactive group and the binding site of the monoclonal antibody 4E. © 1989 Springer-Verlag. |
| Keywords: | human cell; animal; mice; transfection; monoclonal antibody; molecular cloning; cloning, molecular; gene mapping; antibodies, monoclonal; amino acid sequence; molecular sequence data; genetic engineering; epitope; hla-c antigens; immunoblotting; base sequence; hla b antigen; hla c antigen; hla-b antigens; hla antigens; normal human; peptide mapping; epitopes; isoelectric focusing; dna probes; precipitin tests; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Immunogenetics |
| Volume: | 29 |
| Issue: | 1 |
| ISSN: | 0093-7711 |
| Publisher: | Springer |
| Date Published: | 1989-01-01 |
| Start Page: | 25 |
| End Page: | 32 |
| Language: | English |
| DOI: | 10.1007/bf02341610 |
| PUBMED: | 2461903 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
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