Molecular analysis of the serologically defined HLA-Aw19 antigens: A genetically distinct family of HLA-A antigens comprising A29, A31, A32, and Aw33, but probably not A30 Journal Article
| Authors: | Kato, K.; Trapani, J. A.; Allopenna, J.; Dupont, B.; Yang, S. Y. |
| Article Title: | Molecular analysis of the serologically defined HLA-Aw19 antigens: A genetically distinct family of HLA-A antigens comprising A29, A31, A32, and Aw33, but probably not A30 |
| Abstract: | The HLA-Aw19 complex consists of a number of serologically cross-reactive Ag (i.e., A29, A30, A31, A32, and Aw33) which exhibit an epitope shared by HLA-B and -C proteins. To investigate the structural basis for these serologic cross-reactivities, we have cloned and determined the nucleotide sequences for A30, A31, and Aw33, and compared the predicted amino acid sequences with those already available for A29, A32, and other class I allelic products. All alleles of the Aw19 group contained A-locus-specific sequences, exhibiting 'A-ness.' The structural similarities between Aw19 polypeptides were found in the α1 and α2 domains, where shared amino acid residues were identified that correlated with observed serologically reactivity patterns. Seven Aw19-specific nucleotides were found. Two of these were silent substitutions, but the remaining five resulted in Aw19-specific amino acid residues. Each of the HLA-A alleles can be classified into one of the five serologically cross-reacting groups. In the Aw19 group, the alleles A29, A31, A32, and Aw33 are closely related serologically as well as genetically whereas A30 probably belongs to the A1/A3/A11 group. The similarity between A30 and the other Aw19 alleles may have resulted from two independent gene conversions affecting exons 2 and 3. Additional mutations or gene conversion-like events in A30 were also noted. It is postulated that gene conversions have played a significant role in the divergence of the Aw19 alleles. However, each serologically cross-reactive Aw19 allotype appears to have arisen directly from a common ancestral allele. A30 was the only exception, and this allele may represent an unusual allotype, which is subject to a high rate of genetic changes, as is seen in the H-2Kb gene of the mouse. |
| Keywords: | unclassified drug; human cell; molecular genetics; alleles; cloning, molecular; gene conversion; amino acid sequence; molecular sequence data; genetic engineering; hla-c antigens; base sequence; serology; hla-a antigens; hla-b antigens; multigene family; epitopes; cross reactions; genes, mhc class i; human; priority journal; article; allotype; support, u.s. gov't, p.h.s.; hla aw19 antigen |
| Journal Title: | Journal of Immunology |
| Volume: | 143 |
| Issue: | 10 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1989-11-15 |
| Start Page: | 3371 |
| End Page: | 3378 |
| Language: | English |
| PUBMED: | 2478623 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
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