Phosphorylation and down-regulation of CD4 and CD8 in human CTLs and mouse L cells Journal Article
| Authors: | DiSanto, J. P.; Klein, J. S.; Flomenberg, N. |
| Article Title: | Phosphorylation and down-regulation of CD4 and CD8 in human CTLs and mouse L cells |
| Abstract: | The CD4 and CD8 molecules are rapidly phosphorylated following exposure of CD4+ or CD8+ human cytotoxic T lymphocytes (CTL) clones to B-lymphoblastoid cell lines bearing the relevant target alloantigens. Treatment of CD4+ or CD8+ CTL clones with phorbol myristate acetate (PMA), phytohemagglutinin, or mitogenic combinations of CD2-specific antibodies also resulted in CD4 or CD8 phosphorylation. Down-regulation of the surface expression of these molecules could be demonstrated in both CD4+ and CD8+ clones following exposure to the relevant alloantigen or PMA. Parallel experiments were conducted using mouse L cells in which the human CD4 or CD8 antigens were stably expressed. Exposure of these transfectants to PMA induced rapid phosphorylation of the CD4 and CD8 molecules. As in CD4+ CTL clones, rapid modulation of the CD4 antigen could be demonstrated in L cells following PMA treatment. In contrast, there was no demonstrable down-regulation of the CD8 antigen in PMA-treated CD8+ L cell transfectants. These studies demonstrate a significant differential property of the CD4 and CD8 antigens and suggest that down-regulation of the CD8 antigen may require its expression in a T-cell environment and/or the association of CD8 with the T-cell receptor or other T cell-specific molecules. © 1989 Springer-Verlag. |
| Keywords: | protein phosphorylation; human cell; nonhuman; cd8 antigen; animal cell; mouse; animal; mice; down-regulation; transfection; phosphorylation; animalia; t lymphocyte receptor; lymphocyte activation; cell culture; cytotoxic t lymphocyte; t-lymphocytes, cytotoxic; cd4 antigen; antigens, cd4; antigens, ly; antigens, cd8; tetradecanoylphorbol acetate; antigens, differentiation, t-lymphocyte; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; l cells (cell line); l cell |
| Journal Title: | Immunogenetics |
| Volume: | 30 |
| Issue: | 6 |
| ISSN: | 0093-7711 |
| Publisher: | Springer |
| Date Published: | 1989-12-01 |
| Start Page: | 494 |
| End Page: | 501 |
| Language: | English |
| DOI: | 10.1007/bf02421181 |
| PUBMED: | 2512251 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
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