Cytotoxic and proliferative allospecific T-cell clones contain perforin and mediate anti-CD3-induced cytotoxicity Journal Article
| Authors: | Geisberg, M.; Terry, L. A.; Flomenberg, N.; Dupont, B. |
| Article Title: | Cytotoxic and proliferative allospecific T-cell clones contain perforin and mediate anti-CD3-induced cytotoxicity |
| Abstract: | Some in vitro-generated allospecific T-cell clones can kill target cells bearing specific antigen, whereas others can only proliferate in response to that antigen. The mechanism of target lysis by clones that exhibit antigen-specific cytotoxicity is thought to involve the exocytosis of lytic granules, which contain the pore-forming protein perforin. Here, CD4+, CD8+, and CD4-8- T-cell clones, positive for CD3 and the α/β T-cell receptor, were tested for their ability to lyse the mouse-anti-human CD3 hybridoma OKT3; this hybridoma has been shown to trigger the cytolytic mechanism in cytotoxic T cells regardless of their clonal specificity. We found that all in vitro-generated allospecific T-cell clones can efficiently lyse the OKT3 targets whether or not they can kill alloantigen-bearing lymphoblastoid B-cell line targets. Furthermore, all tested clones contained perforin. The OKT3 hybridoma was not lysed by perforin-negative, CD3+ leukemic T-cell lines or by CD3- NK clones. Thus, the presence of perforin in T-cell clones correlated with their ability to lyse OKT3 targets, but not with their ability to lyse alloantigen-bearing targets. These results demonstrate that T-cell clones that are nonlytic when activated by specific antigen nevertheless contain a complete lytic mechanism and also support the proposed central role in perforin in that mechanism. © 1992. |
| Keywords: | human cell; cd3 antigen; cd8 antigen; lymphocyte proliferation; antigens, cd3; animal; mice; models, biological; cytotoxicity; monoclonal antibody; t lymphocyte receptor; lymphocyte activation; antigen specificity; membrane glycoproteins; lymphocyte clone; cytotoxic t lymphocyte; t-lymphocytes, cytotoxic; immunoblotting; protein induction; perforin; cytolysis; target cell; cytotoxicity, immunologic; cd4 antigen; hybridoma; antigens, cd4; clone cells; alloantigen; lymphoblastoid cell line; exocytosis; antigens, cd8; hybridomas; okt 3; human; priority journal; article; support, u.s. gov't, p.h.s. |
| Journal Title: | Human Immunology |
| Volume: | 35 |
| Issue: | 4 |
| ISSN: | 0198-8859 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1992-12-01 |
| Start Page: | 239 |
| End Page: | 245 |
| Language: | English |
| DOI: | 10.1016/0198-8859(92)90005-8 |
| PUBMED: | 1293087 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 30 July 2019 -- Source: Scopus |
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