Differential expression and regulation of the human CD8α and CD8β chains Journal Article
| Authors: | Terry, L. A.; DiSanto, J. P.; Small, T. N.; Flomenberg, N. |
| Article Title: | Differential expression and regulation of the human CD8α and CD8β chains |
| Abstract: | Abstract: The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T‐cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, α and β. The majority of monoclonal antibodies (mAb) reactive with the human CD8 antigen bind the CD8α chain, while a single mAb, T8/2T8–5H7, has been identified which binds to the CD8 α/β heterodimer. While the two chains of CD8 have been presumed to be coordinately expressed in normal T cells, this is not always the case. Northern blot analysis of a panel of T‐cell leukemias and normal cells demonstrate that CD8α and CD8β are not invariably co‐transcribed and phenotypic analysis of fresh and interleukin 2 (IL‐2) expanded peripheral blood mononuclear cells (PBMC) confirm that the CD8α and CD8β chains are differentially expressed at the cell surface. Four distinct subpopulations of CD8 + cells have been identified based on the expression of CD8 α/α or CD8 α/β complexes: (1) T‐cell receptor (TcR) αβ+ T cells which are CD8 α+/β+ (2) TcR αβ+ T cells which are CD8 α+/β‐; (3) TcR γδ+ T cells which are CD8 α+/β‐ and (4) natural killer (NK) cells which are CD8 α+/β‐. We also demonstrate the down‐regulation of the CD8 α/β heterodimers from the surface of a CD8 + T‐cell clone following treatment with phorbol myristate acetate (PMA) while CD8 α/α homodimers remain on the cell surface. This observation demonstrates that a) a CD8+ T‐cell clone can express both CD8 α/α homodimers and CD8 α/β heterodimers and b) these two complexes do not have identical biological properties. Together, these data suggest that CD8 α/α and CD8 α/β dimers may not subserve identical functions. The differential contribution of these two CD8 complexes should be considered in models of T‐cell‐mediated cytotoxicity and T‐cell activation. Copyright © 1990, Wiley Blackwell. All rights reserved |
| Keywords: | human cell; comparative study; cd8 antigen; antigen expression; t lymphocyte; t-lymphocytes; cell line; down-regulation; transcription, genetic; gene expression regulation; cell culture; neutrophils; rodentia; normal human; antigens, cd8; antigens, differentiation, t-lymphocyte; leukemia, t-cell; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Tissue Antigens |
| Volume: | 35 |
| Issue: | 2 |
| ISSN: | 0001-2815 |
| Publisher: | Wiley-Blackwell Publishing, Inc. |
| Date Published: | 1990-02-01 |
| Start Page: | 82 |
| End Page: | 91 |
| Language: | English |
| DOI: | 10.1111/j.1399-0039.1990.tb01761.x |
| PUBMED: | 2111591 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 January 2020 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
-
234Small -
56Flomenberg -
16Di Santo
Related MSK Work