Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings Journal Article
| Authors: | Zhu, W.; Pirovano, G.; O’Neal, P. K.; Gong, C.; Kulkarni, N.; Nguyen, C. D.; Brand, C.; Reiner, T.; Kang, D. |
| Article Title: | Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings |
| Abstract: | Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 μm and provided microscopy images over a sample area larger than 500 μm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM. © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. |
| Keywords: | chemotherapy; fluorescence; tumors; diagnosis; lateral resolution; drug dosage; histologic analysis; epifluorescence microscopy; selenium compounds; pancreatic tumors; smartphones; epifluorescence microscope; fluorescence signals; low-resource settings; microscopy images |
| Journal Title: | Biomedical Optics Express |
| Volume: | 11 |
| Issue: | 1 |
| ISSN: | 2156-7085 |
| Publisher: | Optical Society of America |
| Date Published: | 2020-01-01 |
| Start Page: | 89 |
| End Page: | 98 |
| Language: | English |
| DOI: | 10.1364/boe.11.000089 |
| PROVIDER: | scopus |
| PMCID: | PMC6968742 |
| PUBMED: | 32010502 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 March 2020 -- Source: Scopus |
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