Potentiation of a polyadenylylation site by a downstream protein-DNA interaction Journal Article
| Author: | Dorsett, D. |
| Article Title: | Potentiation of a polyadenylylation site by a downstream protein-DNA interaction |
| Abstract: | The gypsy retroposon of Drosophila melanogaster contains a sequence that potentiates upstream polyadenylylation sites. In contrast to other sequences that influence poly(A) site use, it appears to operate at the level of the DNA template. Nuclear extracts contained protein that bound to a repeated motif in the DNA. Flies with mutations that reduced transcripts polyadenylylated in the 5' long terminal repeat of gypsy contained less DNA-binding activity than wild type. A change in the repeat motif reduced both protein binding and poly(A) site potentiation. These findings provide evidence that DNA-binding proteins can regulate polyadenylylation sites. |
| Keywords: | mutation; dna-binding proteins; nonhuman; animal cell; animal; gene expression; protein binding; transcription, genetic; animalia; nuclear proteins; molecular sequence data; base sequence; drosophila melanogaster; dna mutational analysis; repetitive sequences, nucleic acid; protein dna interaction; retroposon; arthropoda; dna transposable elements; polyadenylation; melanogaster; priority journal; article; poly a; suppressor of hairy-wing; transposable element; arthropod; gypsy |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 87 |
| Issue: | 11 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1990-06-01 |
| Start Page: | 4373 |
| End Page: | 4377 |
| Language: | English |
| DOI: | 10.1073/pnas.87.11.4373 |
| PUBMED: | 2161539 |
| PROVIDER: | scopus |
| PMCID: | PMC54112 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 January 2020 -- Source: Scopus |
