Neutron and γ-irradiation of bacteriophage M13 DNA: Use of standard neutron irradiation facility (SNIF) Journal Article
| Authors: | Singh, S. P.; Cohen, D.; Dytlewski, N.; Houldsworth, J.; Lavin, M. F. |
| Article Title: | Neutron and γ-irradiation of bacteriophage M13 DNA: Use of standard neutron irradiation facility (SNIF) |
| Abstract: | Monoenergetic neutrons/Calibration/RBE/Bacteriophage survival/DNA damage We describe here the use of the Van de Graaff accelerator as a source of high energy neutrons for biological irradiation. Single-stranded bacteriophage M13 DNA was chosen as the system to determine the relative biological effectiveness of monoenergetic neutrons. A Standard Neutron Irradiation Facility (SNIF) was established using a 3 MV Van de Graaff accelerator. The 2D (d,n)3He nuclear reaction was used to produce neutron fluxes of 3xl08cm-2sec-1yielding dose rates as high as 50 Gy h_1. A detailed description of the neutron source, neutron fluence measurement. dose calculation and calibration arc included. Exposure of single-stranded bacteriophagc M13 DNA to 90 Gy of neutrons reduced survival to 0.18% of the unirradiated value. 500 Gy of γ-rays were required for the same level of killing, and RBE was estimated at 6 based on Do values. Determination of the extent of DNA damage after exposure to cleavage using gel electrophoresis, gave RBE values of 6—8 which was very similar to that observed for bacteriophage survival. The facility described here provides a reproducible source of high energy monoenergetic neutrons and dose levels suitable for experiments designed to measure DNA damage and effects on DNA synthesis. © 1990, Journal of Radiation Research Editorial Committee. All rights reserved. |
| Keywords: | dna damage; radiation exposure; dna viruses; relative biological effectiveness; single stranded dna; dna, single-stranded; relative biologic effectiveness; gamma radiation; gamma rays; dna virus; article; coliphage; support, non-u.s. gov't; fast neutron radiation; fast neutrons; coliphages; dna, single stranded |
| Journal Title: | Journal of Radiation Research |
| Volume: | 31 |
| Issue: | 4 |
| ISSN: | 0449-3060 |
| Publisher: | Oxford University Press |
| Date Published: | 1990-12-01 |
| Start Page: | 340 |
| End Page: | 353 |
| Language: | English |
| DOI: | 10.1269/jrr.31.340 |
| PUBMED: | 2098554 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 January 2020 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work