Detection of Candida antigenuria in disseminated candidiasis by immunoblotting Journal Article
| Authors: | Ferreira, R. P.; Yu, B.; Niki, Y.; Armstrong, D. |
| Article Title: | Detection of Candida antigenuria in disseminated candidiasis by immunoblotting |
| Abstract: | Immunoblotting (Western blotting) was used to detect Candida albicans antigens in urine of 10 patients with disseminated candidiasis who had two or more positive blood cultures. Twelve urine samples were examined; and antigenuria was found in five of six patients with C. albicans infections, in one patient with a mixed Candida infection (including C. albicans), and in one of two patients with C. tropicalis infection. All except one specimen was collected from 2 to 12 days after initiation of amphotericin B therapy. Positive samples showed different numbers of bands in Western blots with an antigen that had an apparent molecular weight of 47,000 in common. This antigen was not found in the urine of patients who had more than 5 days of therapy for candidiasis and who were responding to therapy. The results suggest that Western blotting for C. albicans antigens in urine may be a useful method for the diagnosis of disseminated candidiasis and for evaluating antifungal treatment. |
| Keywords: | human cell; nonhuman; methodology; blotting, western; antigen detection; urinary tract infection; immunoblotting; evaluation studies; drug sensitivity; candidiasis; fungus; candida albicans; fungi; candida; fungus antigen; amphotericin b; candida tropicalis; antigens, fungal; human; priority journal; article |
| Journal Title: | Journal of Clinical Microbiology |
| Volume: | 28 |
| Issue: | 5 |
| ISSN: | 0095-1137 |
| Publisher: | American Society for Microbiology |
| Date Published: | 1990-05-01 |
| Start Page: | 1075 |
| End Page: | 1078 |
| Language: | English |
| PUBMED: | 2191006 |
| PROVIDER: | scopus |
| PMCID: | PMC267871 |
| DOI: | 10.1128/jcm.28.5.1075-1078.1990 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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