Methods for the enzymatic synthesis of tyrosine and phenylalanine labeled with nitrogen-13 Journal Article
| Authors: | Gelbard, A. S.; Cooper, A. J. L.; Asano, Y.; Nieves, E.; Filc-Dericco, S.; Rosenspire, K. C. |
| Article Title: | Methods for the enzymatic synthesis of tyrosine and phenylalanine labeled with nitrogen-13 |
| Abstract: | l-[13N]Tyrosine and l-[13N]phenylalanine were synthesized using immobilized enzymes by two methods. In method 1, [13N]ammonia is converted to l-[13N]glutamate; transamination with p-hydroxyphenylpyruvate yields l-[13N]tyrosine. [13N]Tyrosine is separated from other labeled intermediates on a Poropak Q column. In method 2, phenylalanine dehydrogenase catalyzes the reversible reductive [13N]amination of either phenylpyruvate or p-hydroxyphenylpyruvate to form l-[13N]phenylalanine or l-[13N]tyrosine, respectively. The feasibility of labeling DOPA and tryptophan with 13N was also demonstrated. © 1990. |
| Keywords: | nonhuman; methodology; drug synthesis; tyrosine; isotope labeling; radioisotope; amino acids; enzyme synthesis; phenylalanine; tryptophan; dopa; nitrogen 13; nitrogen radioisotopes; article; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; nitrogen - isotopes; nitrogen - 13; enzymes, immobilized |
| Journal Title: | International Journal of Radiation Applications and Instrumentation. Part A: Applied Radiation and Isotopes |
| Volume: | 41 |
| Issue: | 2 |
| ISSN: | 0883-2889 |
| Publisher: | Elsevier, Inc. |
| Date Published: | 1990-01-01 |
| Start Page: | 229 |
| End Page: | 233 |
| Language: | English |
| DOI: | 10.1016/0883-2889(90)90114-v |
| PUBMED: | 2158948 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
