| Abstract: |
[13N]- and [11C]carbamyl phosphate, l-[ω-13N]citrulline, l-[ureido-11C]citrulline, [carbamyl-13N]- and [carbamyl-11C]carbamyl-l-aspartate were synthesized using carbamyl phosphate synthetase coimmobilized with either aspartate transcarbamylase or ornithine transcarbamylase. Carbamyl l-[13N]aspartate was enzymatically prepared from carbamyl phosphate and l-[13N]aspartate. The tissue distribution of radioactivity in mice after injection of radiolabeled ammonia, carbamyl phosphate or citrulline was studied. The tissue distribution of isotope derived from [13N)carbamyl phosphate and [13N]ammonia were similar, with the exception of liver, brain and pancreas, in which 13NH3 uptake was higher after retroorbital injection. The distribution of label derived from l-[ω-13N]- and l-[ureido-13C]citrulline was similar. Substantial tumor (Sarcoma-180) uptake of label from l-citrulline was observed. © 1985. |
| Keywords: |
nonhuman; animal; mice; mice, inbred strains; isotope labeling; tissue distribution; chromatography, high pressure liquid; aspartic acid; carbamates; carbon isotopes; citrulline; carbon radioisotopes; ornithine carbamoyltransferase; nitrogen radioisotopes; female; priority journal; radioisotope dilution technique; aspartate carbamoyltransferase; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; carbamoyl phosphate c 11; carbamoyl phosphate n 13; carbamoylaspartic acid c 11; carbamoylaspartic acid n 13; citrulline c 11; citrulline n 13; carbamoyl-phosphate synthase (ammonia); carbamyl phosphate
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