Growth inhibition by TGF-β linked to suppression of retinoblastoma protein phosphorylation Journal Article

  


Authors: Laiho, M.; DeCaprio, J. A.; Ludlow, J. W.; Livingston, D. M.; Massagué, J.
Article Title: Growth inhibition by TGF-β linked to suppression of retinoblastoma protein phosphorylation
Abstract: The growth-suppressive function of the retinoblastoma gene product, RB, has been ascribed to the underphosphorylated RB form that prevails during G1 phase in the cell cycle. We show that addition of the paracrine growth inhibitor transforming growth factor β1 (TGF-β1) to Mv1Lu lung epithelial cells in mid to late G1 prevents phosphorylation of RB scheduled for this cell cycle stage and arrests cells in late G1. Expression of SV40 T antigen, a transforming protein that binds underphosphorylated RB, does not block the effect of TGF-β1 on RB phosphorylation but greatly reduces the growth-inhibitory response to TGF-β1. TGF-β1 and RB appear to function in a common growth-inhibitory pathway in which TGF-β1 acts to retain RB in the underphosphorylated, growth-suppressive state. © 1990.
Keywords: protein phosphorylation; dna replication; animal; cell division; cell growth; transforming growth factor beta; cell line; retinoblastoma; transfection; phosphorylation; nuclear proteins; genetic engineering; cell culture; lung; transforming growth factors; simian virus 40; phosphoproteins; cell cycle phase; antigens, polyomavirus transforming; retinoblastoma protein; growth inhibition; interphase; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title: Cell
Volume: 62
Issue: 1
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 1990-07-13
Start Page: 175
End Page: 185
Language: English
DOI: 10.1016/0092-8674(90)90251-9
PUBMED: 2163767
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025326725&doi=10.1016%2f0092-8674%2890%2990251-9&partnerID=40&md5=05b062c20438361bb242f7ad8f344ee7
Notes: Article -- Export Date: 27 January 2020 -- Source: Scopus
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MSK Authors
  1. Joan Massague
    388 Massague
  2. Marikki Laiho
    12 Laiho
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