Control of junB and extracellular matrix protein expression by transforming growth factor-β1 is independent of simian virus 40 T antigen-sensitive growth-inhibitory events Journal Article
| Authors: | Laiho, M.; Rönnstrand, L.; Heino, J.; Decaprio, J. A.; Ludlow, J. W.; Livingston, D. M.; Massagué, J. |
| Article Title: | Control of junB and extracellular matrix protein expression by transforming growth factor-β1 is independent of simian virus 40 T antigen-sensitive growth-inhibitory events |
| Abstract: | Treatment of Mv1Lu mink lung epithelial cells with transforming growth factor-β1 (TGF-β1) prevents phosphorylation of the retinoblastoma susceptibility gene product, RB, in late G1 phase of the cell cycle, which is thought to retain RB in a growth-suppressive state. This effect is paralleled by cell cycle arrest in late G1 (M. Laiho, J. A. DeCaprio, J. W. Ludlow, D. M. Livingston, and J. Massagué, Cell 62:175-185, 1990). Arrest can be prevented by expression of simian virus 40 T antigen, which binds to underphosphorylated RB, presumably blocking its growth-suppressive activity. The response of cells to TGF-β1, however, is complex and includes changes in the levels of expression of genes encoding nuclear transcription factors and extracellular matrix components. To define the relationships among various components of the TGF-β1 response, we have investigated the effect of TGF-β1 on cells whose growth-inhibitory response to this factor is prevented by T antigen. TGF-β1 addition to exponentially growing Mv1Lu cells increased the levels of junB mRNA and of three extracellular matrix proteins: plasminogen activator inhibitor-1, fibronectin, and thrombospondin. Kinetically, the effects on junB and plasminogen activator inhibitor-1 expression occurred faster (half-maximal at 1 to 2 h) than the effects on fibronectin and thrombospondin expression (half-maximal at 6 to 10 h). These effects either preceded or overlapped, respectively, the withdrawal of Mv1Lu cells from the cell cycle. Expression of a transfected T-antigen gene in Mv1Lu cells, however, did not prevent any of these responses to TGF-β1. The results indicate that TGF-β1-stimulated expression of junB and extracellular matrix proteins in Mv1Lu cells can occur independently of the T-antigen-sensitive events that lead to growth arrest. |
| Keywords: | dna-binding proteins; nonhuman; animal cell; animal; mammalia; cell cycle; gene expression; transforming growth factor beta; cell line; transfection; animalia; extracellular matrix; gene expression regulation; rna, messenger; mammal; lung; virus large t antigen; simian virus 40; mink; growth inhibition; thrombospondins; fibronectins; simiae; genes, retinoblastoma; proto-oncogene proteins c-jun; simian virus; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; oncogene c jun; mustela vison; plasminogen inactivators; platelet membrane glycoproteins |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 11 |
| Issue: | 2 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 1991-02-01 |
| Start Page: | 972 |
| End Page: | 978 |
| Language: | English |
| PUBMED: | 1990295 |
| PROVIDER: | scopus |
| PMCID: | PMC359761 |
| DOI: | 10.1128/mcb.11.2.972 |
| DOI/URL: | |
| Notes: | Jkrki Heino's first name is misspelled on the original publication -- Source: Scopus |
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