Negative regulation of G1 in mammalian cells: Inhibition of cyclin E-dependent kinase by TGF-β Journal Article
| Authors: | Koff, A.; Ohtsuki, M.; Polyak, K.; Roberts, J. M.; Massagué, J. |
| Article Title: | Negative regulation of G1 in mammalian cells: Inhibition of cyclin E-dependent kinase by TGF-β |
| Abstract: | Transforming growth factor-β (TGF-β) is a naturally occurring growth inhibitory polypeptide that arrests the cell cycle in middle to late G1 phase. Cells treated with TGF-β contained normal amounts of cyclin E and cyclin-dependent protein kinase 2 (Cdk2) but failed to stably assemble cyclin E-Cdk2 complexes or accumulate cyclin E-associated kinase activity. Moreover, G1 phase extracts from TGF-β-treated cells did not support activation of endogenous cyclin-dependent protein kinases by exogenous cyclins. These effects of TGF-β, which correlated with the inhibition of retinoblastoma protein phosphorylation, suggest that mammalian G1 cyclin-dependent kinases, like their counterparts in yeast, are targets for negative regulators of the cell cycle. |
| Keywords: | nonhuman; animal cell; animal; mammalia; cell cycle; enzyme inhibition; protein kinases; transforming growth factor beta; cell line; enzyme activation; phosphorylation; mammal; cycline; cyclins; retinoblastoma protein; protein kinase; g1 phase; cell extracts; mink; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Science |
| Volume: | 260 |
| Issue: | 5107 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 1993-04-23 |
| Start Page: | 536 |
| End Page: | 539 |
| Language: | English |
| DOI: | 10.1126/science.8475385 |
| PUBMED: | 8475385 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
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