| Abstract: |
In a United States series of 28 gastric/esophageal adenocarcinomas of poorly to moderately differentiated histopathology, we detected gene amplification in 21% of the tumors. Using the modified DNA in-gel renaturation assay to detect down to 7–8 copies of amplified DNA sequences, we identified 3 gastric tumors with amplified DNA sequences and confirmed by Southern hybridization analysis that HER-2/neu was amplified <5-fold in these specimens. Immunohistochemical staining of tumor tissue sections with pl85 HER-2/neu antibodies demonstrated that only the 3 gastric adenocarcinomas with corresponding HER-2/neu gene amplification displayed membrane immunoreactivity. Amplification of c-met was identified in 2 tumors, and this was the first study to assay for multiple copies of this protooncogene in fresh gastric tumor tissues. Amplification of c-erbB was observed in one tumor, and no evidence for amplification of i#if-2 was obtained in this series of adenocarcinomas. © 1990, American Association for Cancer Research. All rights reserved. |
| Keywords: |
clinical article; human cell; adenocarcinoma; gene amplification; oncogenes; gene expression regulation, neoplastic; dna, neoplasm; histochemistry; stomach carcinoma; stomach neoplasms; esophagus carcinoma; esophageal neoplasms; oncogene neu; blotting, southern; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
|
