Chromogenic in situ hybridization for the detection of HER-2/neu gene amplification in breast cancer with an emphasis on tumors with borderline and low-level amplification: Does it measure up to fluorescence in situ hybridization? Journal Article

  

Authors:	Bhargava, R.; Lal, P.; Chen, B.
Article Title:	Chromogenic in situ hybridization for the detection of HER-2/neu gene amplification in breast cancer with an emphasis on tumors with borderline and low-level amplification: Does it measure up to fluorescence in situ hybridization?
Abstract:	We compared chromogenic in situ hybridization (CISH) with fluorescence in situ hybridization (FISH) for assessing HER-2/neu gene amplification using tissue microarrays (TMAs) made from formalin-fixed, paraffin-embedded tissue blocks from 113 cases of invasive breast carcinoma. TMAs were created using 0.6-mm tissue cores with 4 sampled cores per tumor. For both assays, a HER-2/chromosome 17 signal ratio of 2.0 or more was considered positive for gene amplification. The average ratio of cores from the same tumor was used for determination of gene amplification status of that particular tumor. Of 113 cases, 102 were tested successfully by both assays. The results were concordant in 100.0% of cases (63 amplified; 39 nonamplified). All 22 cases of borderline (ratio, 2.0-2.5) or low-level (ratio, 2.6-3.9) amplification by FISH also showed HER-2 gene amplification by CISH. CISH is as sensitive as FISH in detecting borderline and low-level HER-2 amplification. Reliable recognition of the invasive carcinoma area by light microscopy and preservation of the test slides are added advantages of CISH. CISH performs as well as FISH in the analysis of HER-2 gene amplification in breast cancer and might have advantages in certain situations.
Keywords:	immunohistochemistry; human tissue; major clinical study; microscopy; genetic analysis; sensitivity analysis; reproducibility of results; in situ hybridization, fluorescence; breast cancer; gene amplification; breast neoplasms; cancer invasion; in situ hybridization; fluorescence in situ hybridization; intermethod comparison; tissue array analysis; receptor, erbb-2; reliability; tissue microarray; fish; genes, erbb-2; tissue fixation; chromosome 17; formaldehyde; paraffin; carcinoma, ductal, breast; oncogene neu; c-erbb-2; her-2/neu; chromogenic in situ hybridization; cish; chromogenic compounds
Journal Title:	American Journal of Clinical Pathology
Volume:	123
Issue:	2
ISSN:	0002-9173
Publisher:	Oxford University Press
Date Published:	2005-02-01
Start Page:	237
End Page:	243
Language:	English
DOI:	10.1309/c4pe-bgb9-ln83-0tvl
PUBMED:	15842048
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-13244268437&partnerID=40&md5=0ef7a1ec41382f02bc9c0e5da86004e8
Notes:	--- - "Cited By (since 1996): 37" - "Export Date: 24 October 2012" - "CODEN: AJCPA" - "Source: Scopus"

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