Detection of HER-2/neu gene amplification in breast cancer using a novel polymerase chain reaction/ligase detection reaction technique Journal Article
| Authors: | Nathanson, D. R.; Nash, G. M.; Chen, B.; Gerald, W.; Paty, P. B. |
| Article Title: | Detection of HER-2/neu gene amplification in breast cancer using a novel polymerase chain reaction/ligase detection reaction technique |
| Abstract: | Background Gene amplification is the primary mechanism of HER-2/neu overexpression in breast cancer and is a strong predictor of prognosis. Currently screening for HER-2/neu gene amplification in breast cancer is done by fluorescent in-situ hybridization (FISH), which is accurate but costly and labor intensive. We have evaluated a new PCR (polymerase chain reaction)-based assay for the detection of HER-2/neu gene amplification in human breast cancer. Study design A total of 15 breast cancer cell lines and 14 breast cancer specimens were evaluated. HER-2/neu status of the tumors was evaluated by FISH and then assessed using a quantitative polymerase chain reaction/ligase detection reaction (PCR/LRD) technique. Results Amplification of the HER-2/neu gene was detected in seven cell lines previously reported to have amplification and no amplification was found in any of the six that had been reported not to have amplification. In the assessment of breast specimens the PCR/LDR and FISH assays were in complete agreement. All 10 tumors with amplification by FISH were also amplified by PCR/LDR. Conclusions The PCR/LDR technique successfully detects HER-2/neu gene amplification in clinical breast cancer specimens and shows 100% concordance with FISH. This technique is an accurate and rapid alternative to FISH with the potential for automation and high throughput analysis of HER-2/neu status in breast cancer. © 2003 by the American College of Surgeons. |
| Keywords: | controlled study; human tissue; human cell; genetics; methodology; polymerase chain reaction; accuracy; proto oncogene; breast cancer; gene amplification; analytic method; cancer screening; tumor cells, cultured; breast neoplasms; fluorescence in situ hybridization; cell culture; gene identification; breast tumor; genes, erbb-2; oncogene neu; ligase chain reaction; humans; human; female; priority journal; article; fish = fluorescent in situ hybridization; ldr = ligase detection reaction; pcr = polymerase chain reaction |
| Journal Title: | Journal of the American College of Surgeons |
| Volume: | 197 |
| Issue: | 3 |
| ISSN: | 1072-7515 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2003-09-01 |
| Start Page: | 419 |
| End Page: | 425 |
| Language: | English |
| DOI: | 10.1016/s1072-7515(03)00431-9 |
| PUBMED: | 12946797 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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