| Abstract: |
Human lymphoblastoid cell lines (Daudi, Daudi subclones, Raji and MOLT-4) were compared for sensitivity to the antiproliferative action of a-interferon (IFN-α) and down-regulation of IFN-α receptors. IFN-sensitive and IFN-resistant cell lines have similar numbers (2-4000/cell) of high affinity (20-75 pM) IFN-α receptors. Treatment of IFN-sensitive cells with low concentrations (3-10 pM) of IFN-β results in low receptor occupancy and nearly complete (> 95%) down-regulation of cell surface IFN-α receptors within 5 h. Treatment of resistant cells with higher IFN concentrations (50 pM) only results in partial (≈60%) receptor down-regulation that is directly related to receptor occupancy. Receptor-receptor interactions, induced by IFN-α binding, may account for the enhanced down-regulation of IFN-α receptors in IFN-sensitive cells. Such interactions apparently do not occur in IFN-resistant lymphoblastoid cell lines. © 1990, American Association for Cancer Research. All rights reserved. |
| Keywords: |
human cell; alpha interferon; cells, cultured; cell division; down-regulation; drug resistance; cell culture; radioisotope; lymphocytes; interferon receptor; receptors, immunologic; growth inhibition; interferon type i; raji cell; lymphoblastoid cell; receptors, interferon; human; priority journal; article; daudi cell; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
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