Interferon α induces the expression of retinoblastoma gene product in human Burkitt lymphoma Daudi cells: Role in growth regulation Journal Article
| Authors: | Kumar, R.; Atlas, I. |
| Article Title: | Interferon α induces the expression of retinoblastoma gene product in human Burkitt lymphoma Daudi cells: Role in growth regulation |
| Abstract: | Interferon α (IFN-α) is a regulatory secretory protein with distinctive biological effects such as antiproliferative actions against many tumor cell lines, including human Burkitt lymphoma Daudi cells. The mechanism underlying growth inhibition by IFN-α is not well established. The growth of many mammalian cell types is also regulated by tumor suppressor retinoblastoma (RB) gene product, the RB protein. In the studies presented here, we explored the possible involvement of RB protein in the growth inhibitory action of IFN-α in the Daudi cell model system. We observed that IFN-α induces a 3- to 10-fold increased expression of RB protein in growth-sensitive Daudi cells but not in the growth-resistant variant of Daudi cells. IFN-α-mediated induction of RB protein was an early event that preceded the period of growth inhibition of Daudi cells. IFN-α-induced RB protein predominantly exists as the underphosphorylated form. Addition of antibody against IFN-α to Daudi cells resulted in the inhibition of constitutive expression of RB protein and stimulation of [3H]thymidine incorporation. These results demonstrate that the induction of RB protein expression in IFN-α-treated Daudi cells could constitute an important mechanism of IFN-α-mediated growth regulation. |
| Keywords: | human cell; alpha interferon; mammalia; cell division; gene expression; cell growth; cell line; gene product; retinoblastoma; tumor cells, cultured; phosphorylation; gene expression regulation; oncogene; rna, messenger; growth regulation; immunoblotting; retinoblastoma protein; burkitt lymphoma; in vitro; cell lysate; growth inhibition; rna analysis; growth inhibitors; growth suppression; interferon alfa-2a; genes, retinoblastoma; human; priority journal; article; daudi cell; support, non-u.s. gov't |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 89 |
| Issue: | 14 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1992-07-15 |
| Start Page: | 6599 |
| End Page: | 6603 |
| Language: | English |
| DOI: | 10.1073/pnas.89.14.6599 |
| PUBMED: | 1631162 |
| PROVIDER: | scopus |
| PMCID: | PMC49549 |
| DOI/URL: | |
| Notes: | Source: Scopus |
