Human renal cancers resistant to IFN's antiproliferative action exhibit sensitivity to IFN's gene-inducing and antiviral actions Journal Article
| Authors: | Pfeffer, L. M.; Wang, C.; Constantinescu, S. N.; Croze, E.; Blatt, L. M.; Albino, A. P.; Nanus, D. M. |
| Article Title: | Human renal cancers resistant to IFN's antiproliferative action exhibit sensitivity to IFN's gene-inducing and antiviral actions |
| Abstract: | Purpose: Although treatment with interferon-alpha (IFN alpha) results in tumor regression in a subset (<20%) of patients with renal cell carcinoma, the underlying mechanisms for the resistance of renal cancer (RC) cells to IFN alpha is unknown. Materials and Methods: We examined 5 RC lines resistant and 5 RC lines sensitive to the antiproliferative effects of IFN alpha for differences in: 1) the number of IFN binding sites, 2) the number of signal-transducing IFNAR-1 chains of the IFN alpha receptor, 3) IFN alpha receptor structure, 4) IFN-stimulated gene (ISG) expression and 5) IFN alpha sensitivity in antiviral assays. Results: No structural alterations in the IFN alpha receptor were detected in any RC line examined, although varying numbers of ligand binding sites and IFNAR-1 signal transducer chains were present. All 5 IFN-sensitive, and 4 of 5 IFN-resistant RC lines were sensitive to the antiviral and gene-inducing actions of IFN alpha. Conclusions: The resistance of RC lines to IFN's antiproliferative action is not due to defects in ligand binding or in IFN-receptor structure. Our results indicate that the defective antiproliferative response in most RC cells is not due to their failure to induce the gene-inducing and antiviral effects of IFN alpha. |
| Keywords: | phosphorylation; carcinoma, renal cell; carcinoma; transcription; expression; cell-lines; factor; protein-tyrosine kinase; interferons; interferon-alpha/beta receptor |
| Journal Title: | Journal of Urology |
| Volume: | 156 |
| Issue: | 5 |
| ISSN: | 0022-5347 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1996-11-01 |
| Start Page: | 1867 |
| End Page: | 1871 |
| Language: | English |
| ACCESSION: | WOS:A1996VM11700111 |
| DOI: | 10.1016/s0022-5347(01)65555-1 |
| PROVIDER: | wos |
| PUBMED: | 8863634 |
| Notes: | Source: Wos |
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