Pharmacokinetics of aerosol amphotericin B in rats Journal Article

Authors: Niki, Y.; Bernard, E. M.; Schmitt, H. J.; Tong, W. P.; Edwards, F. F.; Armstrong, D.
Article Title: Pharmacokinetics of aerosol amphotericin B in rats
Abstract: The distributions of amphotericin B (AmB) in tissue were compared after intraperitoneal or aerosol administration. Rats were sacrificed 24 h after receiving single or repeated daily doses; AmB concentrations in tissues were determined by high-performance liquid chromatography. After intraperitoneal doses of 4 mg/kg of body weight per day for 7 days, mean concentrations of AmB were 122.7, 55.2, and 4.31 μg/g in the spleen, liver, and lung, respectively. After aerosol doses (aero-AmB) of 1.6 mg/kg per day, the mean concentrations of AmB in the lung were 2.79 μg/g after a single dose and 9.88 μg/g after four doses, while the drug was undetectable (< 0.1 μg/g) in serum, spleen, liver, kidney, and brain. The half-life of elimination of AmB from the lungs was 4.8 days according to serial sacrifices done after a single dose of 3.2 mg of aero-AmB per kg. Treatment with 60 mg of aero-AmB per kg was well tolerated and produced no histopathologic changes in the lungs. The aerosol route was much more efficient than the systemic route in delivering AmB to the lungs, and it limited the accumulation of AmB in other organs. Because AmB is eliminated slowly, infrequent dosing schedules can be used. These pharmacokinetic characteristics and its proven effectiveness in an animal model make aero-AmB a highly promising new method for the prevention of pulmonary aspergillosis. Aero-AmB should also be considered for use as an adjunct to intravenous AmB for treatment of fungal pneumonias.
Keywords: nonhuman; spleen; animal experiment; blood; liver; kidney; drug distribution; brain; rat; lung; high performance liquid chromatography; drug half life; intravenous drug administration; amphotericin b deoxycholate; amphotericin b; priority journal; article
Journal Title: Antimicrobial Agents and Chemotherapy
Volume: 34
Issue: 1
ISSN: 0066-4804
Publisher: American Society for Microbiology  
Date Published: 1990-01-01
Start Page: 29
End Page: 32
Language: English
DOI: 10.1128/aac.34.1.29
PUBMED: 2327759
PROVIDER: scopus
PMCID: PMC171515
Notes: Article -- Source: Scopus
Citation Impact
MSK Authors
  1. William Ping-Yiu Tong
    153 Tong
  2. Donald Armstrong
    189 Armstrong
  3. Edward M Bernard
    41 Bernard
  4. Fitzroy F Edwards
    15 Edwards