Detection of the anti‐Hu antibody in the serum of patients with small cell lung cancer—A quantitative Western blot analysis Journal Article

  

Authors:	Dalmau, J.; Furneaux, H. M.; Gralla, R. J.; Kris, M. G.; Posner, J. B.
Article Title:	Detection of the anti‐Hu antibody in the serum of patients with small cell lung cancer—A quantitative Western blot analysis
Abstract:	We looked for the presence of the anti‐Hu antibody in the sera from 50 normal subjects; 44 patients with small cell lung cancer, not associated with paraneoplastic disease; and 25 patients with small cell lung cancer associated with paraneoplastic sensory neuropathy, encephalomyelitis, or both. Using the avidin‐biotin immunoperoxidase method and a highly sensitive quantitative Western blot analysis, the anti‐Hu antibody was not detected in the 50 normal human sera. Seven of the 44 patients with small cell lung cancer but no paraneoplastic syndrome had detectable levels (average titer, 76 U/ml) of anti‐Hu antibody on Western blot. These levels are significantly lower than the average titer of the 25 patients who had small cell lung cancer and paraneoplastic sensory neuropathy or encephalomyelitis (average titer, 4,592 U/ml). In the group with nonparaneoplastic small cell lung cancer (low anti‐Hu titer) there was a predominance of women (5 women: 2 men), and all patients had “limited” disease when diagnosed. In the antibody‐negative group the sex ratio was 16 women to 21 men and 51% of the patients had “extensive” disease. None of the 7 patients with a low‐titer anti‐Hu antibody developed a paraneoplastic syndrome by the time of writing. The anti‐Hu antibody appears, when present, to be a good marker for small cell lung cancer and, when present at high titer, for small cell lung cancer associated with a paraneoplastic syndrome. Copyright © 1990 American Neurological Association
Keywords:	adult; aged; aged, 80 and over; sensory neuropathy; lung neoplasms; neurons; blotting, western; histochemistry; paraneoplastic syndrome; molecular weight; autoantibodies; antibody; small cell carcinoma; carcinoma, small cell; middle age; encephalomyelitis; paraneoplastic syndromes; human; male; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title:	Annals of Neurology
Volume:	27
Issue:	5
ISSN:	0364-5134
Publisher:	Wiley Blackwell
Date Published:	1990-05-01
Start Page:	544
End Page:	552
Language:	English
DOI:	10.1002/ana.410270515
PUBMED:	2163235
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025361155&doi=10.1002%2fana.410270515&partnerID=40&md5=482abe768d96ae58724bd3e3cfaf776d
Notes:	Article -- Source: Scopus

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