The HuD paraneoplastic proteins shares immunogenic regions between PEM/PSN patients and several strains and species of experimental animals Journal Article

  

Authors:	Smitt, P. S.; Manley, G.; Dalmau, J.; Posner, J.
Article Title:	The HuD paraneoplastic proteins shares immunogenic regions between PEM/PSN patients and several strains and species of experimental animals
Abstract:	Patients with small-cell carcinoma of the lung and paraneoplastic encephalomyelitis develop antibodies (anti-Hu) against HuD, a member of a family of neuronal specific RNA-binding proteins, which are also expressed by the tumor. In order to determine if the human HuD paraneoplastic antigen shares immunogenic regions between patients and several strains and species of animals, we immunized animals with HuD and several deletion constructs of this protein. All immunized animals developed high titers of anti-HuD antibodies, comparable to the antibody titers found in paraneoplastic patients. Using immunohistochemistry and Western blot analysis of human and mouse cerebral cortex, the pattern of reactivity of these antibodies could not be differentiated from the human paraneoplastic anti-Hu antibodies. None of the immunized animals developed neurologic symptoms. Western blot analysis of HuD deletion constructs demonstrated that the first and second RNA binding domains were the main immunodominant regions, and that the animal immune response was both strain and species dependent.
Keywords:	immunohistochemistry; clinical article; controlled study; gene deletion; sequence deletion; nonhuman; mouse; animal; animals; mice; animal experiment; nerve tissue proteins; mice, inbred strains; rna binding protein; enzyme immunoassay; molecular cloning; cloning, molecular; immunoenzyme techniques; rna-binding proteins; immunology; immune response; species specificity; hybrid protein; immunoglobulin g; epitope mapping; immunoblotting; autoantigen; autoantigens; species difference; autoimmunity; mouse strain; autoantibody; paraneoplastic syndrome; nerve protein; autoantibodies; hu antigen; hu paraneoplastic encephalomyelitis antigens; immunization; etiology; paraneoplastic disease; encephalomyelitis; paraneoplastic syndromes; humans; human; female; priority journal; article; anti-hu antibodies; auto-immune disease; experimental animals; sjl mice; elavl4 protein, human; elavl4 protein, mouse
Journal Title:	Journal of Neuroimmunology
Volume:	71
Issue:	1-2
ISSN:	0165-5728
Publisher:	Elsevier Inc.
Date Published:	1996-12-01
Start Page:	199
End Page:	206
Language:	English
DOI:	10.1016/s0165-5728(96)00153-1
PUBMED:	8982120
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030338473&doi=10.1016%2fS0165-5728%2896%2900153-1&partnerID=40&md5=45f111011ede3899bbed58b279621466
Notes:	Article -- Export Date: 22 November 2017 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/119124