Hu antigens: Reactivity with hu antibodies, tumor expression, and major immunogenic sites Journal Article
| Authors: | Manley, G. T.; Smitt, P. S.; Dalmau, J.; Posner, J. B. |
| Article Title: | Hu antigens: Reactivity with hu antibodies, tumor expression, and major immunogenic sites |
| Abstract: | HuD, a human neuronal RNA‐binding protein, was the first identified member of a family of antigens that also includes HuC and Hel‐N1 (Hu antigens). The serum of all patients with anti‐Hu–associated paraneoplastic encephalomyelitis and sensory neuronopathy react with HuD and Hel‐N1, but the reactivity with HuC is unknown. In the current study we examined (1) the reactivity of anti‐Hu sera with HuD, HuC, and Hel‐N1; (2) the expression of HuD, HuC, and Hel‐N1 messenger RNA in small‐cell lung cancer of patients with and those without paraneoplastic encephalomyelitis/sensory neuronopathy; (3) the correlation between anti‐Hu serum reactivity with these three Hu antigens and the type of neurological symptoms; and (4) the major immunogenic sites of HuD. Our findings indicate that all anti‐Hu sera react with HuD, HuC, and Hel‐N1. However, only HuD is expressed in the small‐cell lung cancer tumors, indicating that among the three Hu antigens, HuD appears to play a central role in triggering the anti‐Hu immune response. No differences were identified regarding the reactivity of the anti‐Hu antibodies with HuD, HuC, and Hel‐N1 and the spectrum of neurological symptoms presented by the patients. Study of the major immunogenic sites of HuD resulted in the identification of two major immunodominant regions with at least two distinct epitopes recognized by the serum of all patients with anti‐Hu—associated paraneoplastic encephalomyelitis/sensory neuronopathy. Copyright © 1995 American Neurological Association |
| Keywords: | immunohistochemistry; adolescent; human tissue; aged; sequence deletion; polymerase chain reaction; antigen expression; sensory neuropathy; gene expression; lung neoplasms; nerve tissue proteins; tumor cells, cultured; immunoreactivity; rna-binding proteins; lung small cell cancer; immune response; molecular sequence data; rna, messenger; screening; immunogenicity; base sequence; antibodies; rna, neoplasm; antibody titer; carcinoma, small cell; epitopes; middle age; tissue reaction; encephalomyelitis; dna library; paraneoplastic syndromes; human; male; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Annals of Neurology |
| Volume: | 38 |
| Issue: | 1 |
| ISSN: | 0364-5134 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1995-07-01 |
| Start Page: | 102 |
| End Page: | 110 |
| Language: | English |
| DOI: | 10.1002/ana.410380117 |
| PUBMED: | 7541976 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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