Immunization with the paraneoplastic encephalomyelitis antigen HuD does not cause neurologic disease in mice Journal Article
| Authors: | Sillevis Smitt, P. A. E.; Manley, G. T.; Posner, J. B. |
| Article Title: | Immunization with the paraneoplastic encephalomyelitis antigen HuD does not cause neurologic disease in mice |
| Abstract: | Paraneoplastic encephalomyelitis/paraneoplastic sensory neuronopathy (PEM/PSN) associated with small cell lung cancer is characterized by high serum and CSF titers of anti-neuronal (anti-Hu) antibodies and by intrathecal synthesis of anti-Hu IgG. A pathologic role for the anti-Hu antibodies in PEM/PSN is further suggested by reported intraneuronal accumulation of the antibodies in the nervous system of PEM/PSN patients at autopsy. We immunized SJL/J mice, Lewis rats, and Hartley guinea pigs with purified recombinant HuD fusion protein. In spite of high-titer anti-HuD antibodies, neurologic and pathologic examination of the animals was normal. Apparent uptake of purified IgG by neurons in the brain proved to be artifactual. © 1995 American Academy of Neurology. |
| Keywords: | immunohistochemistry; controlled study; nonhuman; mouse; animals; mice; animal tissue; sensory neuropathy; animal experiment; animal model; nerve tissue proteins; rna-binding proteins; lung small cell cancer; molecular sequence data; hybrid protein; immunoglobulin g; brain; recombinant protein; rat; base sequence; rats; antibodies; paraneoplastic syndrome; neurologic disease; hu paraneoplastic encephalomyelitis antigens; antibody formation; antibody titer; immunization; encephalomyelitis; paraneoplastic syndromes; guinea pig; guinea pigs; humans; female; priority journal; article; cell antibody |
| Journal Title: | Neurology |
| Volume: | 45 |
| Issue: | 10 |
| ISSN: | 0028-3878 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1995-10-01 |
| Start Page: | 1873 |
| End Page: | 1878 |
| Language: | English |
| DOI: | 10.1212/wnl.45.10.1873 |
| PUBMED: | 7477985 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
