The Rad50 coiled-coil domain is indispensable for Mre11 complex functions Journal Article
| Authors: | Hohl, M.; Kwon, Y.; Galván, S. M.; Xue, X.; Tous, C.; Aguilera, A.; Sung, P.; Petrini, J. H. J. |
| Article Title: | The Rad50 coiled-coil domain is indispensable for Mre11 complex functions |
| Abstract: | The Mre11 complex (Mre11, Rad50 and Xrs2 in Saccharomyces cerevisiae) influences diverse functions in the DNA damage response. The complex comprises the globular DNA-binding domain and the Rad50 hook domain, which are linked by a long and extended Rad50 coiled-coil domain. In this study, we constructed rad50 alleles encoding truncations of the coiled-coil domain to determine which Mre11 complex functions required the full length of the coils. These mutations abolished telomere maintenance and meiotic double-strand break (DSB) formation, and severely impaired homologous recombination, indicating a requirement for long-range action. Nonhomologous end joining, which is probably mediated by the globular domain of the Mre11 complex, was also severely impaired by alteration of the coiled-coil and hook domains, providing the first evidence of their influence on this process. These data show that functions of Mre11 complex are integrated by the coiled coils of Rad50. © 2011 Nature America, Inc. All rights reserved. |
| Keywords: | controlled study; gene mutation; nonhuman; dna recombination; telomere; mre11 protein; rad50 protein; allele; saccharomyces cerevisiae; double stranded dna break |
| Journal Title: | Nature Structural and Molecular Biology |
| Volume: | 18 |
| Issue: | 10 |
| ISSN: | 1545-9993 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2011-09-04 |
| Start Page: | 1124 |
| End Page: | 1131 |
| Language: | English |
| DOI: | 10.1038/nsmb.2116 |
| PROVIDER: | scopus |
| PMCID: | PMC3190017 |
| PUBMED: | 21892167 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 9 December 2011" - "CODEN: NSMBC" - "Source: Scopus" |
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