The Rad50 hook domain is a critical determinant of Mre11 complex functions Journal Article
| Authors: | Wiltzius, J. J. W.; Hohl, M.; Fleming, J. C.; Petrini, J. H. J. |
| Article Title: | The Rad50 hook domain is a critical determinant of Mre11 complex functions |
| Abstract: | The Mre11 complex (in Saccharomyces cerevisiae: Mre11, Rad50 and Xrs2) influences multiple facets of chromosome break metabolism. A conserved feature of the Mre11 complex is a zinc-coordinating motif in Rad50 called the Rad50 hook. We established a diploid yeast strain, rad50 hook, in which Rad50 is encoded in halves, one from each of the two RAD50 alleles, with the residues constituting the hook deleted. In all respects, rad50 hook phenocopies complete Rad50 deficiency. Replacing the hook domain with a ligand-inducible FKBP dimerization cassette partially mitigated all phenotypes in a ligand-dependent manner. The data indicate that the Rad50 hook is critical for Mre11 complex-dependent DNA repair, telomere maintenance and meiotic double-strand break formation. Sister chromatid cohesion was unaffected by Rad50 deficiency, suggesting that molecular bridging required for recombinational DNA repair is qualitatively distinct from cohesin-mediated sister chromatid cohesion. © 2005 Nature Publishing Group. |
| Keywords: | controlled study; nonhuman; protein function; protein motif; phenotype; telomere; mre11 protein; rad50 protein; complex formation; dna repair; protein protein interaction; chromatid; dna strand breakage; saccharomyces cerevisiae; chromosome breakage; yeast; qualitative analysis |
| Journal Title: | Nature Structural and Molecular Biology |
| Volume: | 12 |
| Issue: | 5 |
| ISSN: | 1545-9993 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2005-05-01 |
| Start Page: | 403 |
| End Page: | 407 |
| Language: | English |
| DOI: | 10.1038/nsmb928 |
| PROVIDER: | scopus |
| PUBMED: | 15852023 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 71" - "Export Date: 24 October 2012" - "CODEN: NSMBC" - "Source: Scopus" |
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