BEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila Journal Article


Authors: Ueberschär, M.; Wang, H.; Zhang, C.; Kondo, S.; Aoki, T.; Schedl, P.; Lai, E. C.; Wen, J.; Dai, Q.
Article Title: BEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila
Abstract: The Drosophila genome encodes three BEN-solo proteins including Insensitive (Insv), Elba1 and Elba2 that possess activities in transcriptional repression and chromatin insulation. A fourth protein—Elba3—bridges Elba1 and Elba2 to form an ELBA complex. Here, we report comprehensive investigation of these proteins in Drosophila embryos. We assess common and distinct binding sites for Insv and ELBA and their genetic interdependencies. While Elba1 and Elba2 binding generally requires the ELBA complex, Elba3 can associate with chromatin independently of Elba1 and Elba2. We further demonstrate that ELBA collaborates with other insulators to regulate developmental patterning. Finally, we find that adjacent gene pairs separated by an ELBA bound sequence become less differentially expressed in ELBA mutants. Transgenic reporters confirm the insulating activity of ELBA- and Insv-bound sites. These findings define ELBA and Insv as general insulator proteins in Drosophila and demonstrate the functional importance of insulators to partition transcription units. © 2019, The Author(s).
Keywords: mutation; nonhuman; gene; gene expression; embryo; protein; drosophila; genetic transcription; rna; chromatin; transgene; binding site; genome; fly; article
Journal Title: Nature Communications
Volume: 10
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2019-12-13
Start Page: 5700
Language: English
DOI: 10.1038/s41467-019-13558-8
PUBMED: 31836703
PROVIDER: scopus
PMCID: PMC6911014
DOI/URL:
Notes: Article -- Source: Scopus
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  1. Eric C Lai
    159 Lai