Converting human pluripotent stem cells to neural tissue and neurons to model neurodegeneration Journal Article

  


Authors: Chambers, S. M.; Mica, Y.; Studer, L.; Tomishima, M. J.
Article Title: Converting human pluripotent stem cells to neural tissue and neurons to model neurodegeneration
Abstract: Human embryonic stem cells (hESCs) and the related induced pluripotent stem cells (hiPSCs) have attracted considerable attention since they can provide an unlimited source of many different tissue types. One challenge of using pluripotent cells is directing their broad differentiation potential into one specific tissue or cell fate. The cell fate choices of extraembryonic, endoderm, mesoderm, and ectoderm (including neural) lineages represent the earliest decisions. We found that pluripotent cells efficiently neuralize by blocking the signaling pathways required for alternative cell fate decisions. In this chapter, we detail methods to direct hESCs or hiPSCs into early neural cells and subsequently postmitotic neurons. © 2011 Springer Science+Business Media, LLC.
Keywords: neurodegeneration; neural; disease modeling; dual smad inhibition; human pluripotent stem cells
Journal Title: Methods in Molecular Biology
Volume: 793
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2011-01-01
Start Page: 87
End Page: 97
Language: English
DOI: 10.1007/978-1-61779-328-8_6
PROVIDER: scopus
PUBMED: 21913095
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-80054774244&partnerID=40&md5=a35b9a895e434278b35613440b0d6880
Notes: Chapter 6 in "Neurodegeneration: Methods and Protocols" (ISBN: 978-1-61779-327-1) --- Export Date: 9 December 2011 - Source: Scopus
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MSK Authors
  1. Lorenz Studer
    220 Studer
  2. Mark J Tomishima
    45 Tomishima
  3. Stuart M Chambers
    15 Chambers
  4. Yvonne Gruber
    6 Gruber
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