Model of recurrent pulmonary aspergillosis in rats Journal Article
| Authors: | Niki, Y.; Bernard, E. M.; Edwards, F. F.; Schmitt, H. J.; Yu, B.; Armstrong, D. |
| Article Title: | Model of recurrent pulmonary aspergillosis in rats |
| Abstract: | Male Sprague-Dawley rats were treated with cortisone acetate and fed a low-protein diet for 3 weeks. At the end of week 2, animals were infected intratracheally with 105 conidia of Aspergillus fumigatus H11-20. Despite discontinuation of steroids and the low-protein diet 1 week after the infection, 94% of controls died of invasive pulmonary aspergillosis within 3 weeks postinfection. When rats were treated with a single dose of 1.6 mg of aerosolized amphotericin B per kg of body weight 48 h prior to the infection, mortality was reduced to 11% within 3 weeks postinfection. Despite apparent good health and rapid weight gain, all survivors showed multiple lesions in histopathological sections of the lungs, and 103 to 104 CFU of aspergilli was recovered from cultures of their lungs. With discontinuation of immunosuppression, the infection was slowly cleared; however, when cortisone acetate was restarted during week 5, reactivation of progressive invasive pulmonary aspergillosis was observed. On the basis of these results, we conclude that a single low dose of aerosolized amphotericin B prophylaxis is effective in preventing an exogenous aspergillus infection of the lung. Additional therapy is needed to prevent recurrent infection caused by endogenous aspergilli when immunosuppression is resumed. |
| Keywords: | controlled study; disease course; histopathology; mortality; nonhuman; animal; animal tissue; animal experiment; animal model; recurrence; recurrent infection; time factors; animalia; chronic disease; immune tolerance; rat; rats; disease models, animal; immunosuppressive treatment; antibiotic prophylaxis; aspergillus; aspergillus fumigatus; aspergillosis; lung aspergillosis; colony forming unit; lung parenchyma; experimental infection; cortisone acetate; amphotericin b deoxycholate; amphotericin b; rats, inbred strains; lung diseases, fungal; male; priority journal; article; inhalational drug administration |
| Journal Title: | Journal of Clinical Microbiology |
| Volume: | 29 |
| Issue: | 7 |
| ISSN: | 0095-1137 |
| Publisher: | American Society for Microbiology |
| Date Published: | 1991-07-01 |
| Start Page: | 1317 |
| End Page: | 1322 |
| Language: | English |
| PUBMED: | 1885728 |
| PROVIDER: | scopus |
| PMCID: | PMC270108 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 September 2019 -- Source: Scopus |
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