Toward homogeneous erythropoietin: Non-NCL-based chemical synthesis of the Gln(78)-Arg(166) glycopeptide domain Journal Article

   


Authors: Tan, Z.; Shang, S.; Halkina, T.; Yuan, Y.; Danishefsky, S. J.
Article Title: Toward homogeneous erythropoietin: Non-NCL-based chemical synthesis of the Gln(78)-Arg(166) glycopeptide domain
Abstract: Single erythropoietin (EPO) glycoforms with defined mature oligosaccharide structures and amino acid sequences are essential to elucidate the molecular mechanisms by which carbohydrates exert various physiological and metabolic functions and to explore the possible links between carbohydrates and the prevention or management of diseases. To demonstrate that it is possible to generate EPO even without recourse to cysteine-based native chemical ligation, a concise synthesis of the partially protected EPO fragment (78-166) bearing fully mature N- and O-glycans is described. © 2009 American Chemical Society.
Keywords: protein domain; biomedical research; erythropoietin; oxygen; molecular mechanics; amines; amino acid sequence; medical research; peptide fragments; peptide fragment; protein structure, tertiary; chemical structure; polysaccharide; amino acids; organic acids; polysaccharides; molecular mechanism; protein tertiary structure; glycoprotein; chemical reaction; synthesis; glutamine; synthesis (chemical); carbohydrates; glycan derivative; glycopeptide; glycopeptides; chemical synthesis; cysteine; native chemical ligation; carbohydrate; nitrogen; chemical procedures; arginine; concise synthesis; glycoforms; metabolic function; o-glycans; oligosaccharide structure
Journal Title: Journal of the American Chemical Society
Volume: 131
Issue: 15
ISSN: 0002-7863
Publisher: American Chemical Society  
Date Published: 2009-04-22
Start Page: 5424
End Page: 5431
Language: English
DOI: 10.1021/ja808704m
PUBMED: 19334683
PROVIDER: scopus
PMCID: PMC2765484
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-67849092526&partnerID=40&md5=078b29713209d9cdf4d70ac3412d8319
Notes: --- - "Cited By (since 1996): 17" - "Export Date: 30 November 2010" - "CODEN: JACSA" - "Source: Scopus"
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MSK Authors
  1. Yu Yuan
    11 Yuan
  2. Zhongping Tan
    15 Tan
  3. Shiying Shang
    9 Shang
  4. Samuel J Danishefsky
    539 Danishefsky
  5. Tamara Halkina
    1 Halkina
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