Immunohistochemical detection of γ/δ T lymphocytes in formalin-fixed paraffin-embedded tissues Journal Article
| Authors: | Jungbluth, A. A.; Frosina, D.; Fayad, M.; Pulitzer, M. P.; Dogan, A.; Busam, K. J.; Imai, N.; Gnjatic, S. |
| Article Title: | Immunohistochemical detection of γ/δ T lymphocytes in formalin-fixed paraffin-embedded tissues |
| Abstract: | T lymphocytes can be distinguished based on the composition of the T-cell receptor (TCR) chain in α/β T cells and γ/δ T cells. Correspondingly, α/β lymphomas can be distinguished from γ/δ lymphomas. The latter are rare neoplasms, which are usually confined to particular organs and tissues and carry a dismal prognosis. Until recently, monoclonal antibody (mAb) clone g3.20 to the TCR γ-chain was the reagent of choice for the immunohistochemical detection of γ/δ T cells and lymphomas in standard formalin-fixed paraffin-embedded tissues. However, due to technical problems, mAb g3.20 became recently unavailable. Our attempts to identify another commercially available clone to the TCR γ-chain were unsuccessful. However, we were able to identify a mAb (clone H-41, SC-100289; Santa Cruz, Dallas, TX) to the TCR δ-chain. H-41 works well in immunohistochemistry on paraffin-embedded tissue and comparison with previously stained cases, shows superior immunolabeling to mAb g3.20. H-41 to the TCR δ-chain appears to be a suitable reagent for the replacement of mAb g3.20. |
| Journal Title: | Applied Immunohistochemistry & Molecular Morphology |
| Volume: | 27 |
| Issue: | 8 |
| ISSN: | 1541-2016 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2019-09-01 |
| Start Page: | 581 |
| End Page: | 583 |
| Language: | English |
| DOI: | 10.1097/pai.0000000000000650 |
| PUBMED: | 29517505 |
| PROVIDER: | scopus |
| PMCID: | PMC7790162 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 October 2019 -- Source: Scopus |
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