T-cell receptor-δ expression and γδ+ T-cell infiltrates in primary cutaneous γδ T-cell lymphoma and other cutaneous T-cell lymphoproliferative disorders Journal Article

Authors: Pulitzer, M.; Geller, S.; Kumar, E.; Frosina, D.; Moskowitz, A.; Horwitz, S.; Myskowski, P.; Kheterpal, M.; Chan, A.; Dogan, A.; Jungbluth, A.
Article Title: T-cell receptor-δ expression and γδ+ T-cell infiltrates in primary cutaneous γδ T-cell lymphoma and other cutaneous T-cell lymphoproliferative disorders
Abstract: Aims: The diagnosis of cutaneous γδ T-cell lymphoma (GDTCL) requires the identification of γδ chains of the T-cell receptor (TCR). Our aim in this study was, by using a new monoclonal antibody (mAb) against TCRδ, to evaluate TCRδ expression in formalin-fixed paraffin-embedded (FFPE) skin tissue from TCRγ+ cutaneous T-cell lymphoma (CTCL), and to assess TCRδ expression within a spectrum of other cutaneous lymphoproliferative disorders (CLPDs). Methods and results: Twelve cases (10 patients) with TCRγ+ CTCL and 132 additional CLPD cases (127 patients) were examined, including mycosis fungoides (MF) (n = 60), cutaneous GDTCL (n = 15), subcutaneous panniculitis-like T-cell lymphoma (SPTCL) (n = 11), and CD30+ lymphoproliferative disorder (LPD) (n = 24). Clone H-41 against TCRδ was used on a Leica Bond-3 automated stainer to label FFPE slides. H-41 immunostaining was graded as percentage infiltrate: high (50–100%), moderate (10–49%), and low (0–9%). In TCRγ+ tumours, 12 of 12 (100%) patients showed TCRδ expression comparable to TCRγ expression. No (0%) TCRγ+ cases were negative for TCRδ. In all CLPDs, TCRδ expression was as follows: GDTCL, 16 of 20 cases (14 of 15 patients) high, two moderate, and two low; MF, 0 of 60 cases high, nine moderate, and 51 low; CD30+ LPD, one of 24 cases high, two moderate, and 21 low; and SPTCL, 0 of 11 cases (0 of 9 patients) high, two moderate, and two low. Three MF-like cases and one SPTCL-like case showed high expression; the remainder showed low expression. Conclusions: mAb H-41 against TCRδ matches TCRγ in immunostaining FFPE tissues from GDTCL, supporting H-41 as a replacement for mAb γ3.20. TCRδ expression in our study suggests that the true occurrence of γδ+ non-GDTCL CTCL/CLPD may be lower than suggested by the recent literature. © 2018 John Wiley & Sons Ltd
Keywords: immunohistochemistry; survival; clinical article; human tissue; protein expression; clinical feature; follow up; t lymphocyte; classification; tumor volume; cohort analysis; monoclonal antibody; cutaneous t cell lymphoma; lymphoproliferative disease; lymphocytic infiltration; t-cell lymphoma; mycosis fungoides; qualitative analysis; lymphoproliferative disorders; t lymphocyte receptor delta chain; subcutaneous nodule; human; priority journal; article; subcutaneous t cell lymphoma; immunological techniques
Journal Title: Histopathology
Volume: 73
Issue: 4
ISSN: 0309-0167
Publisher: Wiley Blackwell  
Date Published: 2018-10-01
Start Page: 653
End Page: 662
Language: English
DOI: 10.1111/his.13671
PROVIDER: scopus
PMCID: PMC6153041
PUBMED: 29893430
Notes: Article -- Export Date: 1 November 2018 -- Source: Scopus
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MSK Authors
  1. Melissa P Pulitzer
    132 Pulitzer
  2. Steven M Horwitz
    368 Horwitz
  3. Alison Moskowitz
    151 Moskowitz
  4. Achim Jungbluth
    342 Jungbluth
  5. Patricia Myskowski
    139 Myskowski
  6. Denise Frosina
    84 Frosina
  7. Ahmet Dogan
    176 Dogan
  8. Erica Elaine Syklawer Kumar
    2 Kumar
  9. Alexander Yoshifumi Chan
    4 Chan
  10. Shamir Geller
    24 Geller