Acid treatment of sera from HIV-1-infected children substantially increases the sensitivity of the HIV-1 p24 antigen immunoassay Journal Article
| Authors: | Povolotsky, J.; Polsky, B.; Abrams, E.; Gold, J.; Liu, K. N.; Dedyo, T.; Kanem, N.; Baron, P.; Chein, N.; Armstrong, D. |
| Article Title: | Acid treatment of sera from HIV-1-infected children substantially increases the sensitivity of the HIV-1 p24 antigen immunoassay |
| Abstract: | The relatively low sensitivity of HIV-1 p24 antigen detection in sera of HIV-1-infected individuals decreases the usefulness of HIV-1 p24 antigen as a virological marker of infection. In order to assess the effect of acid hydrolysis of immune complexes on the sensitivity of the HIV-1 p24 antigen immunoassay, we evaluated 111 untreated and acid-treated sera of 43 HIV-1-infected children. HIV-1 p24 antigen was detected in 38 sera (34.2%) before acid treatment and in 88 sera (79.3%) after acid treatment. High levels of HIV-1 p24 antigen (> 100 pg/ml) were detected in 5 untreated sera (4.5%) and in 51 acid-treated sera (45.9%). We conclude that acid treatment may considerably improve the sensitivity of the HIV-1 p24 antigen immunoassay. |
| Journal Title: | Pediatric AIDS and HIV Infection |
| Volume: | 3 |
| Issue: | 3 |
| ISSN: | 1045-5418 |
| Publisher: | Mary Ann Liebert, Inc. |
| Date Published: | 1992-01-01 |
| Start Page: | 108 |
| End Page: | 111 |
| Language: | English |
| ACCESSION: | WOS:A1992JF36800002 |
| PROVIDER: | wos |
| Notes: | Article -- Source: Wos |
