CD34(+) cell content of 126 341 cord blood units in the US inventory: Implications for transplantation and banking Journal Article
| Authors: | Barker, J. N.; Kempenich, J.; Kurtzberg, J.; Brunstein, C. G.; Delaney, C.; Milano, F.; Politikos, I.; Shpall, E. J.; Scaradavou, A.; Dehn, J. |
| Article Title: | CD34(+) cell content of 126 341 cord blood units in the US inventory: Implications for transplantation and banking |
| Abstract: | CD34+ cell dose is critical for cord blood (CB) engraftment. However, the CD34+ content of the CB inventory in the United States is unknown. We examined the CD34+ cell content of 126 341 red blood cell-depleted US units banked from January 2007 to September 2017 with a total nucleated cell (TNC) count of ≥90 × 107 and a cryovolume of 24-55 mL. Median precryopreservation TNC content was 127 × 107 (interquartile range [IQR], 108-156 × 107); CD34+ cell contentwas 44×105 (IQR, 29 to 67×105). Themedian CD341:TNC ratiowas 0.34%. TNC and CD34+ cell content correlation was weak (r 5 0.24). Of 7125 units with TNCs of ≥210 ×107, only 47%had CD34+ content of ≥100×105. However, some units had high CD34+ content for a given TNC count. Only 4%of CB units were acceptable as single-unit grafts (TNCs, ≥2.5×107/kg; CD34+ cells, ≥1.5 × 105/kg) for 70-kg patients; 22% of units were adequate for 70-kg patients using lower dose criteria (TNCs, ≥1.5 × 107/kg; CD34+ cells, ≥1.0 × 105/kg) suitable for a double-unit graft. These findings highlight that units with the highest TNC dose may not have the highest CD34+ dose, units with unexpectedly high CD34+ content (a ratio of .1.0%) should be verified, and the US CB inventory of adequately sized single units for larger patients is small. They also support the ongoing use of double-unit grafts, a focus on banking high-dose units, and development of expansion technologies. © 2019 by The American Society of Hematology. |
| Keywords: | controlled study; human cell; united states; cd34 antigen; standardization; cryopreservation; engraftment; blood bank; medical technology; umbilical cord blood; cell count; erythrocyte; cell nucleus; bone marrow transplantation; procedures concerning cells; cellular parameters; human; priority journal; article |
| Journal Title: | Blood Advances |
| Volume: | 3 |
| Issue: | 8 |
| ISSN: | 2473-9529 |
| Publisher: | American Society of Hematology |
| Date Published: | 2019-04-23 |
| Start Page: | 1267 |
| End Page: | 1271 |
| Language: | English |
| DOI: | 10.1182/bloodadvances.2018029157 |
| PUBMED: | 30995984 |
| PROVIDER: | scopus |
| PMCID: | PMC6482363 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 August 2019 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
-
243Scaradavou -
335Barker -
103Politikos
Related MSK Work