CD34(+) cell content of 126 341 cord blood units in the US inventory: Implications for transplantation and banking Journal Article


Authors: Barker, J. N.; Kempenich, J.; Kurtzberg, J.; Brunstein, C. G.; Delaney, C.; Milano, F.; Politikos, I.; Shpall, E. J.; Scaradavou, A.; Dehn, J.
Article Title: CD34(+) cell content of 126 341 cord blood units in the US inventory: Implications for transplantation and banking
Abstract: CD34+ cell dose is critical for cord blood (CB) engraftment. However, the CD34+ content of the CB inventory in the United States is unknown. We examined the CD34+ cell content of 126 341 red blood cell-depleted US units banked from January 2007 to September 2017 with a total nucleated cell (TNC) count of ≥90 × 107 and a cryovolume of 24-55 mL. Median precryopreservation TNC content was 127 × 107 (interquartile range [IQR], 108-156 × 107); CD34+ cell contentwas 44×105 (IQR, 29 to 67×105). Themedian CD341:TNC ratiowas 0.34%. TNC and CD34+ cell content correlation was weak (r 5 0.24). Of 7125 units with TNCs of ≥210 ×107, only 47%had CD34+ content of ≥100×105. However, some units had high CD34+ content for a given TNC count. Only 4%of CB units were acceptable as single-unit grafts (TNCs, ≥2.5×107/kg; CD34+ cells, ≥1.5 × 105/kg) for 70-kg patients; 22% of units were adequate for 70-kg patients using lower dose criteria (TNCs, ≥1.5 × 107/kg; CD34+ cells, ≥1.0 × 105/kg) suitable for a double-unit graft. These findings highlight that units with the highest TNC dose may not have the highest CD34+ dose, units with unexpectedly high CD34+ content (a ratio of .1.0%) should be verified, and the US CB inventory of adequately sized single units for larger patients is small. They also support the ongoing use of double-unit grafts, a focus on banking high-dose units, and development of expansion technologies. © 2019 by The American Society of Hematology.
Keywords: controlled study; human cell; united states; cd34 antigen; standardization; cryopreservation; engraftment; blood bank; medical technology; umbilical cord blood; cell count; erythrocyte; cell nucleus; bone marrow transplantation; procedures concerning cells; cellular parameters; human; priority journal; article
Journal Title: Blood Advances
Volume: 3
Issue: 8
ISSN: 2473-9529
Publisher: American Society of Hematology  
Date Published: 2019-04-23
Start Page: 1267
End Page: 1271
Language: English
DOI: 10.1182/bloodadvances.2018029157
PUBMED: 30995984
PROVIDER: scopus
PMCID: PMC6482363
DOI/URL:
Notes: Article -- Export Date: 2 August 2019 -- Source: Scopus
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  1. Juliet N Barker
    230 Barker